THE IMPROVING EFFECT OF DANSHENSU ON CEREBRAL MICROCIRCULATION DISORDER AND NEURON DAMAGE AFTER ISCHEMIA-REPERFUSION

Jinfeng Zhang^*

Fourth ward, Tangshan Paraplegia Sanatorium, Tangshan, 063000, China

Objective: The paper aimed to study the effect of Danshensu on cerebral ischemia-reperfusion disorders and neuronal damage. 

Methods: 45 male SD rats were used in the study. An I/R rat model was established by carotid artery occlusion. The rate of albumin leakage in the venules at 20, 40 and 60 min after perfusion was examined using a vertical fluorescence microscope. The number of adhering white blood cells was quantitatively analyzed by observing the cerebral microvessels by He-Ne laser beam. Image Pro Plus software was used to measure the RBC velocity in the venules and the inner diameter of the cerebral venules. Quantitative analysis of rat cerebral infarct volume and nervous system was performed by TTC staining and Longa standard. The morphological changes of rat hippocampal neurons were observed by H&E. TUNEL staining was evaluated by the effect of Danshensu on the apoptosis of hippocampal neurons. 

Results: The albumin leakage rate of the I/R group was higher than that of the control group, and the albumin leakage rate of the Danshensu group was lower than that of the I/R group. The number of leukocyte adhesion in the I/R group was higher than that in the control group, and the number of leukocyte adhesion in the Danshensu group was lower than that in the I/R group. The RBC speed of the I/R group was lower than that of the control group, and the RBC speed of the Danshensu group was higher than that of the I/R group. The venule diameter of the control group was higher than those of the I/R group and Danshensu group. Compared with the control group, the cerebral infarction volume and nerve damage score of the I/R group were higher, and the cerebral infarction volume and nerve damage score of the Danshensu group were lower than those of the I/R group. The rat hippocampus neurons in the I/R group were arranged irregularly, the cell bodies contracted into triangles, and the structure was unclear. The loss of hippocampal neurons in the Danshensu group decreased. The number of apoptosis of cortex and hippocampal neurons in the I/R group increased, and the number of apoptosis of the meridian neurons in the Danshensu group was lower than that of the I/R group. The levels of caspase-3 and Bax in the I/R group were higher, and the levels of Bcl-2 were lower. The levels of Bcl-2 protein expression were higher. 

Conclusion: Danshensu has an obvious protective effect on cerebral I/R injury and its mechanism contributes to the enhancement of cerebral microcirculation and restraining neuronal apoptosis.