MIR-126-3P PROMOTES THE PROLIFERATION AND MIGRATION OF GREAT SAPHENOUS VEIN ENDOTHELIAL CELLS BY REGULATING ERK AND AKT SIGNALING PATHWAYS

Xuepeng Wang^{1, #}, Zhaohui Zhu^{2, #}, Zilin He², Song Zhao², Yulin Wang³, Jingli Tian⁴, Feng Gao^{2, *}

¹Department of Cardiology, Wanfeng Hospital of Shanxi Province, Taiyuan, PR China - ²Department of Cardiology, Beijing Jiangong Hospital, Beijing 100054, PR China - ³Xuchang People's Hospital, Xuchang, PR China - ⁴Beijing Jiangong Hospital, Beijing 100054, PR China

Objective: To analyze the mechanism of miR-126-3p promoting the proliferation and migration of great saphenous vein endothelial cells by regulating ERK and AKT signaling pathways.

Methods: Great saphenous veins were cultured in vitro. A real-time quantitative PCR method was used to detect the expression of miR-126-3p mRNA in these veins. Cultured saphenous vein endothelial cells were divided into miR-126-3p overexpression, miR-126-3p inhibition, set blank, overexpression control, and inhibition control groups. An EdU proliferation experiment was used to detect cell proliferation, and a Transwell experiment was used to analyze cell migration ability. Western blotting was used to determine ERK, AKT, and phosphorylation levels in each group of cells.

Results: miR-126-3p significantly increased on the 7th day of culture in the saphenous vein and decreased to an uncultured level on the 14th day. Compared with the blank group, the miR-126-3p overexpression group significantly promoted the proliferation of the saphenous vein endothelial cells (p<0.05), and the miR-126-3p inhibition group significantly inhibited saphenous vein endothelial cell proliferation (p<0.05). The overexpression control and suppression control groups had no significant effect on the endothelial cell proliferation rate (p>0.05). Compared with the blank group, the miR-126-3p overexpression group significantly promoted great saphenous vein endothelial cell migration (p<0.05), and the miR-126-3p inhibition group significantly inhibited these cells’ migration (p<0.05). Compared with the blank group, the miR-126-3p overexpression group significantly increased the expression of ERK phosphorylation and AKT phosphorylation in saphenous vein endothelial cells (p<0.05), and the miR-126-3p inhibition group significantly inhibited the large. The expression of ERK phosphorylation and AKT phosphorylation in saphenous vein endothelial cells decreased (p<0.05).

Conclusion: Overexpression of miR-126-3p can promote the proliferation and migration of great saphenous vein endothelial cells, and its mechanism may be related to the regulation of ERK and AKT signaling pathways.