EXPRESSION OF HYPOXIA-INDUCIBLE FACTOR-1 Α AND ENOLASE-1 IN LARYNGEAL CARCINOMA AND CORRELATION ANALYSIS

Chong Yan¹, Zhang Liu^{2, *}

¹Department of Otorhinolaryngology, People's Hospital of Three Gorges University, Yichang 443000, Hubei Province, China - ²Affiliated Hospital of Binzhou Medical College, Binzhou 256600, Shandong Province, China

Objective: To investigate the expression of hypoxia-inducible factor-1 α and enolase-1 in laryngeal carcinoma and correlation analysis. 

Methods: A total of 45 male patients with LSCC were selected from the Department of Otorhinolaryngology in our hospital from December 2015 to December 2016. The LSCC tissue and adjacent tissues (more than 0.5 cm from the cancerous tissue) were taken. The immunohistochemical method was used to detect the positive expression rate of HIF-1α and ENO1 in LSCC tissue and adjacent tissues, and further analyze the correlation between the two in LSCC tissue. 

Results: The positive expression rate of HIF-1α in LSCC tissues was 75.55%, which was significantly higher than the positive expression rate of HIF-1α in adjacent tissues of 22.22% (P<0.05). The positive expression rate of ENO1 in LSCC tissues—80.00%—was significantly higher than that of ENO1 in adjacent tissues: 22.22% (P<0.05). The expression level of HIF-1α was correlated with lymph node metastasis and differentiation in LSCC patients (P<0.05). This was unrelated to the patient's age, daily smoking habits, and clinical stage (P>0.05). ENO1 was correlated with lymph node metastasis and differentiation in LSCC patients (P<0.05). This was also unrelated to the patient's age, smoking habits, and clinical stage (P>0.05). After correlation analysis, HIF-1α and ENO1 were found to be positively correlated in LSCC tissues (P<0.05, r=0.591).

Conclusions: The expressions of HIF-1α and ENO1 in LSCC tissues are both up-regulated, and their expression levels are correlated with the degree of lymph node metastasis and differentiation in LSCC patients. In addition, HIF-1α and ENO1 are positively correlated in LSCC tissues, and the two are expected to become potential biomarkers of LSCC, providing important references for clinical evaluation and disease prevention.