EFFECTS OF HEMATOXYLIN EXTRACT ON SERUM INTERLEUKIN, HYPERSENSITIVE C-REACTIVE PROTEIN, AORTIC TLR4 MRNA AND NF-ΚB P65 MRNA IN ATHEROSCLEROSIS MODEL RATS

Jianfei Yang¹, Lai Wei¹, Qianzhu Jiang², Ziyi Guo³, Zengguang Fan³, He Wang^{1, *} 

¹First Affiliated Hospital, Heilongjiang University of Chinese Medicine - ²Department of Cardiology, The Second Affiliated Hospital of Heilongjiang University of traditional Chinese Medicine - ³Heilongjiang University of Chinese Medicine

Objective: To investigate the effects of hematoxylin extract on serum interleukin, hypersensitive C-reactive protein, aortic TLR4 mRNA and NF-κB p65 mRNA in atherosclerosis model rats. 

Methods: Vitamin D3 combined with high-fat feed was used to prepare an atherosclerosis rat model. According to the random number method, 60 rats were divided into normal control group, model group, hematoxylin extract low dose group (containing hematoxylin ethyl acetate extract 1.15g), hematoxylin extract medium dose group (hematoxylin ethyl acetate Ester extract 2.30g), hematoxylin extract high dose group (hematoxylin ethyl acetate extract 3.45g), simvastatin group. The levels of interleukin-1 (IL-6) and hypersensitive C-reactive protein (hs-CRP) were measured by enzyme-linked immunosorbent assay; the expression of TOLL-like receptor 4 (aortic TLR4 mRNA) was measured by real-time fluorescent quantitative PCR; Real-time fluorescent quantitative PCR method was used to detect the mRNA level of nuclear factor κB (NF-κB) p65. 

Results: Compared with the normal control group, the levels of IL-6, hs-CRP, TLR4 mRNA and NF-κB p65 mRNA in the model group were significantly increased (P<0.05). Compared with the model group, the levels of IL-6, hs-CRP, TLR4 mRNA, and NF-κB p65 mRNA of hematoxylin extracts in the low, medium and high dose groups and simvastatin group were significantly reduced (P<0.05). Compared with the simvastatin group, there was no significant difference in the levels of IL-6, hs CRP, TLR4 mRNA and NF- κB p65 mRNA between the low, medium, and high dose groups of hematoxylin extract. 

Conclusion: Hematoxylin extract can inhibit the body's inflammatory response, protect the vascular endothelium and resist the formation of atherosclerosis, and can be used as a candidate drug for AS treatment.