EFFECTS OF CYPA ON PROLIFERATION, MIGRATION, AND INVASION OF COLORECTAL CANCER CELLS THROUGH ERK/MAPK SIGNAL TRANSDUCTION PATHWAY

Xiaonan Dai^#, Wencai Weng^{#, *}, Yadong Meng, Yubing Sun, Mingzhi Ren

Department of Interventional Therapy, Xinhua Hospital Affiliated to Dalian University, Dalian 116000, Liaoning Province, China

Objective: To investigate the effects of cyclophilin A (CyPA) on the proliferation, migration, and invasion of colorectal cancer cells through the ERK / MAPK signal transduction pathway. 

Methods: Human colorectal cancer cell line HCT116 and normal colorectal mucosa cells were cultured in vitro, and were then transfected with CyPA overexpression vector (CyPA overexpression group) and interference vector (CyPA interference group), respectively. Western blot was used to detect the expression of CyPA protein in normal colorectal cells and colorectal cancer cells; CCK-8 method was used to detect the proliferation ability of each group; Transwell test was used to detect the migration and invasion ability of each group; Western blot method was also used to detect the expression of ERK / MAPK signaling pathway-related proteins.

Results: The expression of CyPA protein in HCT116 cells was significantly higher than in normal colorectal cells (P<0.05). Compared with the blank control group, the proliferation ability of HCT116 cells in the CyPA overexpression group was significantly increased at 24 h, 48 h, and 72 h, while the cell proliferation ability of the CyPA interference group was significantly decreased (P<0.05). Compared with the blank control group, the number of migration and invasion cells in the CyPA overexpression group was significantly increased, while the number of migration and invasion cells in the CyPA interference group was significantly decreased (P<0.05). Compared with the blank control group, the expression of CD147 and p-ERK1 / 2 protein in the CyPA overexpression group was significantly increased, while the expression of CD147 and p-ERK1/2 protein in the CyPA interference group was significantly decreased (P<0.05). 

Conclusion: CyPA is highly expressed in colorectal cancer cells, which can promote cell proliferation, migration, and invasion. The related mechanism may be achieved by regulating the ERK/MAPK signal transduction pathway.