MICRORNA-485-5P RESTRAINS MIGRATION AND INVASION OF SPINAL OSTEOSARCOMA CELLS BY INHIBITING FRIZZLED7

Hui Lan¹, Qingsong Tian², Huihui Xiong², Chenglan Ye^1*

¹Department of Medical Oncology, Lishui Municipal Central Hospital - ²Department of Orthopedics, Affiliated Renhe Hospital of China Three Gorges University

Objective: Although osteosarcoma is the second largest primary bone tumor, it is rarely involved with the spine. Spinal involvement accounts for 3 to 5 percent of all osteosarcomas. MicroRNAs control diverse cellular functions by negatively modulating the expression of genes. The purpose of the current work was to investigate microRNA-485-5p role in spinal osteosarcoma and its underlying mechanism.

Methods: The correlation between microRNA-485-5p expression and clinical and pathologic features in spinal osteosarcoma were observed. Real-time PCR(RT-PCR) and western blotting assays were used to probe the expressions of related mRNA and proteins. Luciferase reporter assay was applied to analyze the target gene of microRNA-485-5p.Cell migration and invasion were observed in U2OS and MG-63 cells after treatment with microRNA-485-5p mimics. 

Results: In spinal osteosarcoma tissues and the patients with metastasis, the expression of microRNA-485-5p decreased significantly. Treatment with microRNA-485-5p mimics reduced cell migration and invasion. Frizzled-7 (FZD7) is one of the microRNA-485-5p target gene predicted by bioinformatics and confirmed by luciferase reporter assays. In addition, over-expression of microRNA-485-5p reduced FZD7 expression in MG-63 cells. 

Conclusions: MicroRNA-485-5p possesses effects against migration and invasion in spinal osteosarcoma cells partially via inhibiting FZD7, which could be a promising therapeutic agent for spinal osteosarcoma.