Minxia Qiu, Cancan Kong, Wenmei Chen, Wei Mao*
Endoscopic diagnosis and treatment center, Hainan Provincial People’s Hospital, Haikou 570311, Hainan Province, China
This study aimed to explore the roles and molecular mechanisms of Ki-67 and microRNA-27a (miR-27a) in organ-specific gastric cancer (GC) metastasis to further clarify the mechanism of GC metastasis and provide a theoretical basis for identifying new therapeutic targets of advanced GC. We collected and processed GC samples, which then underwent microRNA extraction and immunohistochemical staining. Following the assessment of Ki-67 and miR-27a expression in GC tissues and normal gastric mucosa samples, we analyzed the relationships between Ki-67 and miR-27a expression in GC patients and gender, age, tumor location, size, general type, differentiation degree, invasion depth, lymph node metastasis, clinical department, as well as the relationship between clinical and biological behaviors. Ki-67 positive signals were primarily expressed in the nuclei of normal gastric tissue. Among 10 normal gastric tissues, 5 were Ki-67 negative, 4 were weakly positive, 1 was moderately positive, and 0 were strongly positive. In 80 GC tissues, 24 were Ki-67 negative, 12 were weakly positive, 18 were moderately positive, and 26 were strongly positive. Ki-67 expression increased gradually with the increase of tumor focus, differentiation degree, invasion depth, lymph node metastasis, clinical division, and the malignant degree of GC. It demonstrated a higher and faster growth rate and gradually increased aggression and transferability.
Ki-67, microRNA-27a, gastric cancer patients, depth of infiltration.