MIR-3142 ACTIVATES PI3K/AKT SIGNALING PATHWAY THROUGH TARGETED REGULATION OF PTEN EXPRESSION TO MEDIATE THE PROLIFERATION OF CHRONIC MYELOID LEUKEMIA CELLS

Zhenxiu Shan¹, Qingfang Zeng^{2, *}

¹Department of Oncology, Gongan County People's Hospital, Jingzhou 434300, Hubei Province, China - ²Department of Internal Medicine Area Three, Ganzhou Cancer Hospital, Ganzhou 341000, Jiangxi Province, China

Chronic myelogenous leukemia (CML) is a malignant cell proliferative tumor disease in the bone marrow. It is also commonly known as blood cancer. This disease has a very low cure rate and is one of the killers of humans. Mirna can suppress oncogenes and is closely related to the occurrence and development of tumors. MiR-3142 plays a role in the treatment of chronic myeloid leukemia by regulating its target genes. The purpose of this article is to further explore the specific situation of miR-3142 stimulating PI3K/Akt signaling pathway to mediate the proliferation of chronic myeloid leukemia cells through targeted regulation of PTEN expression. Ten healthy persons who were confirmed by comprehensive physical examination were taken as the reference group. The two groups of patients were injected with miR-3142 reagent. Fluorescence quantitative PCR was used to detect the difference of miR-3142 in the two groups of patients. Flow cytometry was used to detect the expression level of PTEN. The effect of target gene PTEN on the molecular expression of PI3K/Akt signaling pathway was used to detect the specific situation of cell proliferation of chronic myeloid leukemia cells under the action of miR-3142. The results showed that under the control of miR-3142, the expression level of the target gene PTEN was down-regulated from the original (86.31±4.36) to (57.28±4.02), and the decrease of PTEN expression level could reverse the miR-3142 to PI3K/Akt signaling pathway. Activation, the proliferation index of chronic myeloid leukemia K562 cells decreased from the original (266.43±8.29) to (183.52±6.76). It can be seen that miR-3142 down-regulates PTEN expression and activates the PI3K/Akt signaling pathway under reversal. MiR-3142 can effectively down-regulate the proliferation of chronic myeloid leukemia cells through the PI3K/Akt signaling pathway.