EFFECT AND MECHANISM OF VITAMIN B6 COMBINED WITH NERVE GROWTH FACTOR ON INTELLIGENCE OF CEREBRAL PALSY RATS WITH ISCHEMIA AND HYPOXIA

Kangling Liu^1#, Xueyan Lin^2#, Jing Wang^1#, Rong Wu^1*

¹Department of Pediatrics, Wuhan No.5 Hospital, Wuhan 430050, Hubei Province, China - ²Department of Infection, Wuhan No.5 Hospital, Wuhan 430050, Hubei Province, China

Objective: To study the effect and mechanism of vitamin B6 (VB6) combined with mouse nerve growth factor (mNGF) on the intelligence of cerebral palsy rats with ischemia and hypoxia. 

Methods: 36 SPF grade 7-day-old SD rats were selected and divided into sham operation group, model group and intervention group, with 12 rats in each group. The model group and intervention group established the cerebral palsy model of ischemia and hypoxia. In the sham operation group, only the left common carotid artery was isolated. The intervention group was injected with VB6 and mNGF under the epineurial membrane after the model was established. Sham operation group and model group were given the same dose of normal saline. Once a day for 3 weeks. Observe and record the general condition observation. The memory function of the young rats in each group was detected, the pathological changes of the brain were observed by HE staining, the apoptosis rate was detected by flow cytometry, and the apoptosis related proteins Bcl-2, Bax, Caspase3 and nerve growth related proteins NSE, MAP-2, MBP and other proteins in the brain tissue of each group were detected by Western blot. 

Results: the time taken by the model group from turning on the light to escaping to the safe area was significantly higher than that of the sham operation group, while the time taken by the intervention group was significantly lower than that of the model group (p<0.05). In the model group, the structure of the cerebral cortex was scattered, the number of neurons decreased, nerve cells atrophied, cells were arranged irregularly, and the nuclear color deepened and became pyknotic, while the intervention group was better than the model group. The apoptosis rate of nerve cells in the intervention group was significantly lower than that in the model group (P<0.05). The level of Bcl-2 protein in the intervention group was significantly higher than that in the model group, and the levels of Bax and Caspase3 protein were significantly lower than that in the model group (P<0.05). The expression level of NSE in the intervention group and model group was significantly higher than that in the sham operation group, but the level of NSE in the intervention group was lower than that in the model group (P<0.05). There was no significant difference in the expression level of MAP-2 between the intervention group, the model group and the sham operation group (p>0.05). The expression level of NSE in the intervention group and the model group was significantly higher than that in the sham operation group, and the NSE level in the intervention group was higher than that in the model group (P<0.05). 

Conclusion: VB6 combined with mNGF can improve the intelligence of young rats with cerebral palsy caused by ischemia and hypoxia, and its mechanism may be to inhibit apoptosis by regulating the expression of apoptosis related proteins Bcl-2, Bax, Caspase3. At the same time, it promotes the expression of nerve growth related proteins NSE, MAP-2 and BMP, and improves the self-healing ability of nerve cells.