EFFECT OF TRANSFORMING GROWTH FACTOR-Β 1 ON ECM EXPRESSION AND CELL BEHAVIOR OF AIRWAY SMOOTH MUSCLE CELLS IN PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE

Weiguo Xu^{#, *}, Junhua Wu^#, Hui Li, Jing Zhu, Chongmei Yue

Department of Respiratory and Critical Care Medicine, Mianyang Central Hospital of Sichuan Province, Mianyang 621000, PR China

Objective: To analyze the effect of transforming growth factor-β1 (TGF-β1) on the expression of extracellular matrix (ECM) and cell behavior of airway smooth muscle cells in patients with chronic obstructive pulmonary disease (COPD). 

Methods: Airway smooth muscle cells isolated from COPD patients were cultured in vitro and treated with TGF-β1(10ng/ml). The expression levels of chemokine-1/8 (CXCL-1/8), interleukin-6 (IL-6), matrix metalloproteinase-10, 12 (MMP-10, 12), fibronectin and collagen-8 α 1 (col8 α 1) were detected by ELISA after TGF-β1 stimulated airway smooth muscle cells; the effects of TGF-β1 on the adhesion and repair function of airway smooth muscle cells were observed. 

Results: There was no significant difference in the expression of cxcl-1 between the two groups (P>0.05). The expression of CXCL-8 and IL-6 in the TGF-β1 group was significantly higher than that in the control group (P<0.05). The expression of MMP-3 and MMP-10 in TGF-β1 group was significantly higher than that in control group (P<0.05). The expression of fibronectin and col8 α 1 protein in airway smooth muscle cells in TGF-β1 group was significantly higher than that in control group (P<0.05). Compared with the control group, TGF-β1 group significantly enhanced the adhesion and repair ability of airway smooth muscle cells (P<0.05). 

Conclusion: TGF-β1 can significantly increase the expression of extracellular matrix protein in COPD patients, which has a tendency to improve the adhesion of airway smooth muscle cells and wound repair ability of COPD patients, which provides reference for clinical treatment of COPD.