Huan Yang1, 2, San-Qiang Li1, 2, *, Lei-Na Wang3, Meng-Li Yang1, 2, Bing-Bing Zhang1, 2, Xiao-Min Hong1, 2, Ren-Li Luo1, 2, Hua Fan4, Dan Wang4, Ping Wang1, 2, *, Dong-Mei Wang1, 2, *
1The molecular medicine key laboratory of liver injury and repair, School of Basic Medical Sciences, Henan University of Science and Technology, Luoyang - 2Henan Center for Engineering and Technology Research on Prevention and treatment of liver Diseases, Luoyang - 3Luoyang Polytechnic, Luoyang - 4The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, People’s Republic of China
Introduction: To explore the role of ADAMTS-1 in alcoholic liver fibrosis in mice, and to provide theoretical targets in clinical research and treatment of alcoholic liver fibrosis.
Materials and methods: CRISPR/Cas9 technology was used to inhibit the expression of ADAMTS-1 gene in the mice which were fed with a Lieber-DeCarli diet that contained 5% alcohol by mass for 8 weeks. Then, the effect of ADAMTS-1 on liver fibrosis was explored through histopathology and western blotting.
Results: Inhibiting the expression of ADAMTS-1 promotes the progress of alcoholic liver fibrosis in mice.
Conclusion: Inhibition of ADAMTS-1 aggravated the process of alcoholic liver fibrosis by promoting the overexpression of various pro-injury factors and the activation of TGF-b/Smad3 signaling pathway. Which means that ADAMTS-1 has a protective effect on alcoholic liver fibrosis in mice.
ADAMTS-1, alcohol, liver fibrosis.