THE PROTECTIVE ROLE OF ADAMTS-1 IN ALCOHOLIC LIVER FIBROSIS

Huan Yang^{1, 2}, San-Qiang Li^{1, 2, *}, Lei-Na Wang³, Meng-Li Yang^{1, 2}, Bing-Bing Zhang^{1, 2}, Xiao-Min Hong^{1, 2}, Ren-Li Luo^{1, 2}, Hua Fan⁴, Dan Wang⁴, Ping Wang^{1, 2, *}, Dong-Mei Wang^{1, 2, *}

¹The molecular medicine key laboratory of liver injury and repair, School of Basic Medical Sciences, Henan University of Science and Technology, Luoyang - ²Henan Center for Engineering and Technology Research on Prevention and treatment of liver Diseases, Luoyang - ³Luoyang Polytechnic, Luoyang - ⁴The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, People’s Republic of China

Introduction: To explore the role of ADAMTS-1 in alcoholic liver fibrosis in mice, and to provide theoretical targets in clinical research and treatment of alcoholic liver fibrosis. 

Materials and methods: CRISPR/Cas9 technology was used to inhibit the expression of ADAMTS-1 gene in the mice which were fed with a Lieber-DeCarli diet that contained 5% alcohol by mass for 8 weeks. Then, the effect of ADAMTS-1 on liver fibrosis was explored through histopathology and western blotting. 

Results: Inhibiting the expression of ADAMTS-1 promotes the progress of alcoholic liver fibrosis in mice. 

Conclusion: Inhibition of ADAMTS-1 aggravated the process of alcoholic liver fibrosis by promoting the overexpression of various pro-injury factors and the activation of TGF-b/Smad3 signaling pathway. Which means that ADAMTS-1 has a protective effect on alcoholic liver fibrosis in mice.