Peng Lv1, Xiaocui Ji2, Dawei Qin3, Mingsi Yang1, Baiji Xue1, *
¹School of Basic Medical Sciences, Baicheng Medical College, Baicheng 137000, China - 2Quality Monitoring and Evaluation Office, Baicheng Medical College, Baicheng 137000, China - 3College of Traditional Chinese Medicine, Baicheng Medical College, Baicheng 137000, China
Introduction: To study the inhibitory effect of Scutellarin(SCU) on proliferation of glioma C6 cells and to explore its mechanism.
Materials and methods: Then C6 cells were cultured and divided into control and 0, 5, 10, 20, 40, 80 mg·L-1 SCU groups. The morphology of C6 cells was observed by inverted microscope; The survival rates of C6 cells was examined by MTT assay; The apoptotic rates of C6 cells were examined by Annexin V/PI staining; The expression of Caspase-3 and Caspase-9 proteins in C6 cells were detected by Western blotting.
Results: The MTT results showed that compared with levels blank control group, the survival rates of C6 glioma cells in SCU groups were decreased. The results of inverted microscope observation showed that the cell number in 80 mgL-1 SCU group was significantly decreased, and the cell volume was smaller. The C6 cells were shrinkage, shedding, contour blur, adherent cell outline. The results of AnnexinV/PI staining showed that the late and total apoptotic rates of C6 cells in 80 mg·L-1 SCU group were increased compared with Blank control group. The western blotting results showed that compared with the control group, the expression levels of caspase-3 and caspase-9 protein in 20,40 and 80 mg·L-1 SCU groups were significantly increased.
Conclusion: Compared with the blank control group, SCU can up-regulate the expression of caspase-3 and caspase-9 protein, inhibit tumor growth and induce apoptosis.
Scutellarin, glioma, caspase-3, proliferation.