EFFECTS OF CD147 AND MMP-9 ON THE INVASIVENESS OF SALIVARY GLAND TUMOURS

Yue Yin¹, Yang Wang¹, Lili Zhao¹, Yuan Yao1, Yinghui Li^{2, *}

¹Department of Stomatology, North China University of Science and Technology Affiliated Hospital, Tangshan 063000, Hebei Province, China - ²Department of Orthodontics, School and Hospital of Stomatology, Hebei Medical University & Hebei Key Laboratory of Stomatology, Shijiazhaung 050017, Hebei Province, China

Objective: To analyse the effects of protease inducible factor (CD147) and matrix metalloproteinase-9 (MMP-9) on the invasiveness of salivary gland tumours. 

Methods: Some salivary gland tumour tissue specimens that were surgically removed in the oral pathology department of our hospital between January 2020 and December 2020 were selected as the observation group. The normal salivary gland tissue of patients with parotid gland trauma and sublingual cysts in our hospital was selected as the control group. The patients in the observation group were divided into benign and malignant groups according to the pathological examination results. The malignant group was divided into metastatic and nonmetastatic groups according to the presence or absence of cancer cell metastasis. Patients with adenoid cystic carcinoma (ACC) were divided into nerve invasion and non-nerve invasion groups according to the presence or absence of nerve invasion. The expressions of MMP-9 and cdl47 were detected using pv-9000 two-step immunohistochemistry. A Kappa test was used to evaluate the consistency of CD147 and MMP-9 expression. 

Results: The positive rates of CD147 and MMP-9 in salivary gland tumours in the benign and malignant groups were significantly higher than those in the control group; the positive rates of CD147 and MMP-9 in salivary gland tumours in the malignant group were significantly higher than those in the benign group (P<0.05). The positive rates of CD147 and MMP-9 expression in salivary gland tumours in the metastatic group were significantly higher than those in the nonmetastatic group (P<0.05). The positive rates of CD147 and MMP-9 in salivary gland tumours in the nerve invasion group were significantly higher than those in the non-nerve invasion group (P<0.05). The negative, weak positive, positive and strong positive expression rates of CD147 and MMP-9 in salivary gland tumours were 41.82%, 7.27%, 40.91% and 3.64%, respectively; the expression consistency was 93.64%. A Kappa test was used to evaluate the consistency of the expression of CD147 and MMP-9. A Kappa value close to 0.785 indicated good consistency. 

Conclusion: The expression of CD147 and MMP-9 in salivary gland tumours is consistent. The high expression of CD147 and MMP-9 is closely related to the invasion and nerve invasion of salivary gland malignant tumours.