Dioscorea septemloba thunb granules decreases serum uric acid level in hyperuricemia rat via regulating Oatp1a1 and Octn2 levels

Yujie Lin^a#, Xiaolin Chen^b#, Aizhen Pan^a, Ke Chen^a,  Guiyun Wu^*c, Shijun Zhang^*a

^aDepartment of Traditional Chinese Medicine, Sun Yat-sen Memorial Hospital Affiliated to Sun Yat-sen University, Guangzhou, 510120, China - ^bUniversity Town Hospital of Guangdong Hospital of traditional Chinese Medicine, Integrative Dept 1, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, 510000, China- ^cDepartment of Anesthesiology, Sun Yat-sen Memorial Hospital Affiliated to Sun Yat-sen University, Guangzhou, 510120, China

Purpose: The purpose of this study was to explore the effect of DST granules on the serum uric acid (UA) level in HUA rats.

Methods: The rat hyperuricemia (HUA) model was established by administrating adenine (200 mg/kg/d) for 21 days. The HUA rats were given DST at 120 mg/kg/d (low-dose group), 240 mg/kg/d (mild-dose group), or 480 mg/kg/d (high-dose group) for 14 days by oral administration. At the same time, normal rats and HUA rats were given equivalent 15% CMC-Na. The mRNA and protein expression levels of ATPase Na+/K+ transporting subunit alpha 1 (ATP1a1) and organic cation/carnitine transporter 2 (OCTN2) were evaluated by PCR, Western blot, and immunohistochemical staining.

Results: DST significantly decreased the HUA-induced elevation of serum UA, Cr, and BUN levels (P < 0.05). The serum UA level was decreased by DST in a dose-dependent manner (P < 0.05). DST clearly attenuated HUA induced decreased levels of OATP1a1 and OCTN2 in the liver, kidney, stomach, and small intestine tissues in a dose-dependent manner (P < 0.05).

Conclusion: DST may decrease serum levels of UA, Cr, and BUN through up-regulation of OATP1a1 and OCTN2 in the liver, kidney, stomach, and small intestine tissues.