DIFFERENTIATION- AND INVASION-RELATED EFFECTS OF THE RAB5A GENE ON HUMAN LUNG ADENOCARCINOMA CELL LINES GLC-82 AND SPC-AL

Ge Wen^{1, *}, Qunfeng Hu², Huifang Deng²

¹Department of Radiation Oncology, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Guangdong, China - ²Department of Oncology, Jinshazhou Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510068, Guangdong, China

Objective: The purpose of this article is to investigate the effects of the RAB5A gene on the differentiation and invasion characteristics of two human lung adenocarcinoma cell lines, GLC-82 and SPC-al.

Methods: The RAB5A antiretroviral recombinant (pcdna3-AntiRAB5A) prepared in this paper and the expression receptor RAB5A were transfected with human lung adenocarcinoma GLC-82 and low metastatic human lung adenocarcinoma in poorly differentiated cell lines. In the SPC aluminium electrolytic cell series, after a stable selection, we used experiments with nude mice and the in vitro invasion of alkaline artificial membranes and chemotherapy to observe the differentiated cells and their transformation characteristics after transfection. 

Results: The experimental results in this article showed the invasion ability of GLC-82 cells to the recombinant alkaline membrane in vitro, and its chemical mobility, to have been reduced to 13% and 21%, respectively, after transfection. The contaminated GLC-82 cells displayed a basal-like structure, and the degree of differentiation increased by 25%. After contamination, the ability of SPC-al cells to classify chemical substances, and to invade and reorganise basement membranes, increased by 15%. 

Conclusion: The RAB5A gene plays an important role in the formation of the invasion and metastasis phenotype of GLC-82 and SPC-al cells, and in the differentiation of GLC-82 cells.