SIMULTANEOUS DETERMINATION OF SIX COMPONENTS IN DALBERGIA BENTHAMii PRAIN. BY GAMES

Ge Song^{# 1,2.3}, Chun Hua He^#4, Qing Xia Huang^1,2.3, Chuan Xian Qin^1,2.3, Jian Hua Wei^1,2.3, Chen Yan Liang^*1,2.3, Xu Feng^*1,2,3

Ge Song^{# 1,2.3}, Chun Hua He^#4, Qing Xia Huang^1,2.3, Chuan Xian Qin^1,2.3, Jian Hua Wei^1,2.3, Chen Yan Liang^*1,2.3, Xu Feng^*1,2,3

Introduction: Determining the six components of Dalbergia Benthamii Prain. using quantitative analysis of multi-components by a single marker (QAMS).

Materials and methods: A HPLC method was developed as QAMS to determine glycyrrhizic acid, robustic acid, 4'-hydroxy-5,7-dimethoxy-6-(3-methyl-2-butenyl)-isoflavone, robustin methyl ether, methyl robustate, and 18α-glycyrrhetinic acid in Dalbergia Benthamii Prain. Glycyrrhizic acid was selected as the internal reference substance, and the relative correction factors of robustic acid, 4'-hydroxy-5,7-dimethoxy-6-(3-methyl-2-butenyl)-isoflavone, robustin methyl ether, methyl robustate, and 18α-glycyrrhetinic acid were calculated. The contents of these six active components were determined using the external standard method and QAMS. The rationality, feasibility, and repeatability of the QAMS method were verified by comparing the results obtained from the two different methods. 

Results: The RCFs of robustic acid, 4'-hydroxy-5,7-dimethoxy-6-(3-methyl-2-butenyl)-isoflavone, robustin methyl ether, methyl robustate, and 18α-glycyrrhetinic acid were 0.1651, 0.1507, 0.3662, 0.1956, and 0.6013, respectively, indicating good reproducibility. There was no significant difference between the results of the external standard method and QAMS. 

Conclusion: The proposed QAMS method was accurate and feasible according to the methodological experiments, which can be used to evaluate the quality of Dalbergia Benthamii Prain.