Xiaozhu Zha1, Mei Kang2, Liyang Zhu2, Dong Chen3, Yichun Wang2*
1Department of Traditional Chinese Medicine, Anqing Medical and Pharmaceutical College, Anqing, P. R. China - 2Department of Radiation Oncology, the First Affiliated Hospital of Anhui Medical University, Hefei, P. R. China - 3Center for Genome Analysis, Wuhan Ruixing Biotechnology Co., Ltd., Wuhan, P. R. China
The upregulation of nuclear expression of S100 calcium-binding protein A4 (S100A4) is associated with aggressive behavior and poor survival in cancers. However, the underlying molecular events regulated by nuclear S100A4 is poorly understood. Here, we performed improved RNA immunoprecipitation sequencing (iRIP-seq) to identify S100A4-RNA interaction map within Hela cells. Our results revealed that S100A4 binding targets were enriched in 5'UTR regions, 3'UTRregions and coding DNA sequence regions, and there were well overlapped peaks in two replicates, suggesting that S100A4 had relatively stable RNA binding activity. S1004-RNA interaction associated genes were enriched in cancer-related functional pathways, such as regulation of alternative RNA processing and DNA repair. S100A4-binding gene associated mRNAs and LncRNAs were commonly up-graduated and played important roles in malignant tumors. Moreover, many mRNAs were involved in regulation of DNA damage and repair. In conclusion, S100A4-RNA interaction can modulate the progression and metastasis of cancer through complicated interaction with binding targets. We firstly analyzed the whole transcriptome of S100A4-overexpressed expression, which adds to the understanding of the critical role of S100A4 in malignant tumors.
S100A4, RNA-seq, DNA repair, RNA binding protein, transcriptome analysis.