Haiying Li, Yanfang Fu, Hongyun Sun, Na Ren, Rongzhen An, Xiumin Wang*
Department of Dermatology, The Affiliated Hospital of Binzhou Medical University, Binzhou 256600, PR China
Objective: The aetiology of vitiligo is still unclear, and it is difficult to cure.
Method: There are many clinical treatments for vitiligo, but their clinical efficacy varies because of individual differences. Therefore, the detection of regulatory B cells in the peripheral blood of patients with vitiligo has far-reaching significance. To detect the number of B cells and regulatory B cells in peripheral blood and peripheral blood mononuclear cells (PBMCs) of patients with vitiligo and the expression of surface molecular markers, and to analyse the effect of regulatory B cells. The subjects of this experiment were 15 patients with non-segmental progressive vitiligo. Fifteen normal people were uses as the control group. The expression of B cells in peripheral blood was detected by flow cytometry. The expression of CD19 + B cells, CD19+IL-10+B cells, CD19+CD5highCD1dhighB cells and CD19+CD24highCD38highB cells were detected after the PBMCs were cultured and stimulated.
Results: The percentage of CD24+CD5highCD38highB cells in patients with vitiligo was (3.77± 1.11) %, while in the control group it was (1.13± 0.62) %. The difference between the two groups was statistically significant (P<0.05).
Conclusion: The detection of regulatory B cells in the peripheral blood of vitiligo patients in this paper has achieved good analysis results.
Vitiligo patients, regulatory B cells, microscopy, flow cytometry.