Bing Yu*


Department of Cardiothoracic Surgery, Medical Community General Hospital, Fenghua District People's Hospital, Ningbo 315500, Zhejiang, China


Objective: The surface symptoms of respiratory Mycoplasma infection are similar to other common diseases, so patients with early-stage respiratory Mycoplasma infection are easily misdiagnosed and the optimal treatment time is missed.

Method: This paper explores the utility of microscopy in the diagnosis of early respiratory Mycoplasma infection. We divided 54 patients into two groups to test the positivity rate. A group of 24 patients were tested with the rapid liquid culture of Mycoplasma commonly used in clinics. The remaining 30 patients were tested with ultra-high power microscopy. In addition, Mycoplasma pneumoniae and M. pneumoniae DNA, antibody IgG and IgM, WBC, N%, LYM% and Hs-CRP values were examined with ultra-high power microscopy.

Results: The overall positivity rate was 82.87% in the ultra-high power microscopy group and 36.73% in the Mycoplasma rapid liquid culture group and the false positivity rate of LYM% was the highest at 30.2%. Other indicators were similar. Compared by accuracy, WBC and Hs-CRP were higher, up to 50.6%, and N and LYM were 44% and 42.1%, respectively. Therefore, ultra-high power microscopy is more accurate at detecting Mycoplasma than rapid liquid culture, especially in the early stage of infection.

Conclusion: Although there is no significant difference in effectiveness for later detection, microscopy is better than the conventional rapid liquid culture of Mycoplasma for early detection.


Ultra-high power microscope, respiratory mycoplasma infection, microbial culture, K-means cluster analysis.