CONFOCAL LASER MICROSCOPE OBSERVATION OF BACTERIAL BIOFILM FORMATION AND PATHOGENIC ANALYSIS ON THE SURFACE OF TRACHEAL TUBES OF NEONATES RECEIVING MECHANICAL VENTILATION

Tieyan Wang^{1, *}, Liyan Zhao², Haijun Wang³, Dongying Wu¹, Liyan Wu²

¹Department of Pediatrics, the Second Affiliated Hospital of Qiqihar Medical College, Qiqihar 161000, Heilongjiang, China - ²Department of Neonatal, Qiqihar Hospital of Traditional Chinese Medicine, Qiqihar 161005, Heilongjiang, China - ³Department of Neurology, the First Affiliated Hospital of Qiqihar Medical College, Qiqihar 161042, Heilongjiang, China

Objective: The purpose of this article is to explore the observation and pathogenic analysis of bacterial biofilm formation on the surface of the tracheal tubes of mechanically ventilated neonates by laser confocal microscopy, as well as to conduct field research.

Methods: The test subjects were 30 mechanically ventilated neonates admitted to a hospital affiliated with a medical university between September 2015 and March 2016.

Results: The study demonstrated that 7 types of pathogenic bacteria could be detected on the surface of the tracheal tubes of mechanically ventilated neonates, of which Staphylococcus aureus accounted for 27%, Enterobacter cloacae accounted for 20% and Stenotrophomonas melophilia accounted for 3%. With the extension of the length of mechanical ventilation of neonates, the probability of neonatal lower respiratory tract infections continues to increase. In addition, different mechanical ventilation methods affect the probability of infection. Patients with pulmonary tracheal ventilation have the lowest probability of infection, the probability increases with oral tracheal ventilation, and patients with nasal tracheal ventilation have the highest probability of infection.

Conclusion: The use of laser confocal microscopy can be effective for observing the formation and determining the number of bacterial biofilms on the surface of neonatal tracheal tubes. Furthermore, this technique can be used to carry out effective pathogenic analysis.