CLINICAL VALUE OF LNC RNA UCA1 IN THE DIAGNOSIS OF ACUTE MYOCARDIAL INFARCTION AND ITS CORRELATION WITH MIR-1 EXPRESSION

Bin Hou^{1, #, *}, Liping Li^{2, #}

¹Department of Geriatrics, Suining Central Hospital, Suining 629000, PR China - ²Department of Dermatology, The First People's Hospital of Suining, Suining 629000, PR China 

Objective: to analyse the clinical value of long non-coding RNA (lncrna) urothelial carcinoma Xiangguang 1 (UCA1) in the diagnosis of acute myocardial infarction (AMI) and its correlation with the expression of miR-1. 

Methods: 86 patients with AMI admitted to our hospital between January 2019 and January 2020 were selected as the observation group, while 40 healthy people from the physical examination centre of our hospital were selected as the control group. 3ml of venous blood was collected from AMI patients at 0-2h, 3-6h, 7-12h, 13-24h, 25-48h and 49-72h. 3ml of venous blood was collected from the healthy people during physical examination. The levels of UCA1 and MIR1 were detected through real-time quantitative PCR. The value of UCA1 in the diagnosis of AMI was analysed using C-curve. Pearson linear correlation was used to analyse the correlation between UCA1 and MIR1. The objective was to analyse the clinical value of LNC RNA UCA1 in the diagnosis of AMI and its correlation with miR-1 expression.

Results: the expression of UCA1 in AMI patients decreased significantly 3-24 hours after MI, reaching the lowest level at 7-12 hours, but the difference was statistically significant (P<0.05). The expression level of UCA1 in AMI patients increased gradually from 13 to 72 hours, but it was still lower than that of the control group; the difference was statistically significant (P<0.05). ROC curve analysis showed that the AUC of UCA1 in diagnosing AMI was 0.689, sensitivity was 68.52%, specificity was 69.34%; AUC of diagnosing AMI was 0.758, sensitivity was 76.38%, specificity was 78.95%; AUC of diagnosing AMI at 12-24h was 0.812, sensitivity was 82.64%, specificity was 85.17%; AUC of diagnosing AMI at 25-48h was 0.741, sensitivity was 0.741 At 49-72h after MI, the AUC of AMI was 0.695, the sensitivity was 63.59%, and the specificity was 69.85%. The expression level of MIR1 in AMI patients increased significantly 3-12h after MI and reached the highest level at 7-12h, but the difference was statistically significant (P<0.05); the expression level of miR-1 in AMI patients decreased gradually from 13 to 72 hours, and the difference was statistically significant (P<0.05). There was no significant correlation between UCA1 and MIR1 at 0-2h (P>0.05); there was significant negative correlation between UCA1 and MIR1 at 3-6h, 7-12h, 13-24h, 25-48h and 49-72h (r = -0.426, - 0.638, - 0.416, - 0.587, - 0.526; P<0.05 or <0.01). 

Conclusion: the expression of UCA1 in AMI patients after myocardial infarction is clearly abnormal; it changes with the change of infarct time and has a significant negative correlation with MIR1 expression. It has certain value in the diagnosis of AMI and can be widely used in clinical practice.