TUBEIMOSIDE-1 INHIBITED INVASIVENESS OF PC-3 CELLS THROUGH DEACTIVATE NLRP3 INFLAMMASOME

Zhu Ke¹, Zhang Chao^{2, *}

¹Urology Surgery department, Chengdu Jin Niu District People's Hospital, Chengdu, Sichuan Province 610036, China - ²The first people's Hospital of En Yang District, Bazhong City, Sichuan Province, 636600, China

Prostate cancer is the most frequently diagnosed urinary tract tumor. Tubeimoside-1(TBMS-1) plays an important role in inducing prostate cancer cells apoptosis and cell cycle arrest. However, the effect of TBMS-1 on the prostate cancer cells invasiveness has never been studied. In our study, PC-3 cells were treated with 0, 0.1, 1.0, and 10.0 µM TBMS-1 for 12, 24, and 48 hours, then Nigericin and LPS, were added to active NLRP3 signaling. The cell viability and invasiveness were detected in PC-3 cells. The expression of inflammasome component proteins (NLRP3, Caspase-1, IL-1β) and MMP-2 were detected by Western blotting. Our study showed that TBMS-1 treatment significantly decreased the of PC-3 cells’ viability and invasiveness (P<0.05). The inflammasome component protein (NLRP3, Caspase-1, IL-1β) and MMP-2 were significantly decreased after TBMS-1 exposure (P<0.05), which was nullified by Nigericin (P<0.05). In conclusion, we found that TBMS-1 inhibited the viability and invasiveness of PC-3 cells. We also found that TBMS-1 exposure significantly suppressed the expression of MMP-2 and inflammasome component proteins, which would be abrogated by Nigericin + LPS exposure. All of these proved that deactivation of NLRP3 inflammasome may serve as an important step in TBMS-1 induced PC-3 cell invasiveness reduction.