THE PROTECTIVE EFFECT OF PUERARIN ON RETINA OF DIABETIC RATS AND INHIBITION OF NF - Κ B EXPRESSION

Zhirui Li¹, Yuan Tian^2*, Cong Ren³, Fangrui Liu⁴

¹College of Management, Shandong University of Traditional Chinese Medicine, Jinan, 250355, China - ²College of Foreign Languages, Shandong University of Traditional Chinese Medicine, Jinan, 250355, China - ³College of Ophthalmology and Optometry, Shandong University of Traditional Chinese Medicine, Jinan, 250014, China - ⁴College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 250355, China

Objective: To investigate the protective effect of Puerarin on the retina of diabetic rats and the inhibition of NF - κ B expression.

Method: The diabetic retinopathy model was established in rats. Thirty rats were randomly divided into the puerarin group (10 rats), the model group (10 rats), and the normal group (10 rats). Model group: the same amount of saline was injected once a day for 20 days. Normal group: normal feeding. Puerarin group: rats were intraperitoneally injected with Puerarin 10 mg / kg once a day for 20 consecutive days. The general condition, body weight, blood glucose, protein expression and other related indicators were observed and analyzed. Finally, the data were statistically analyzed. In general, there was no significant difference in the initial body weight among the three groups.

Results: The body weight of the normal group increased faster than that on the third day after injection (P < 0.001), and the difference gradually increased. There was no significant difference in blood glucose among the three groups, but it was statistically significant compared with the normal group (P < 0.001). Compared with the model group, the expression of PKC protein in the retina of Puerarin group was significantly lower than that of the model group (P < 0.01). In the comparison of curative effect, the recovery, obvious effect, and effective combination were combined. The remission effect of Puerarin group was better than that of the model group.

Conclusion: Puerarin can improve vision and hemorheology. Puerarin can protect the diabetic retina by inhibiting PKC activation and NF - κ B expression and reduce the expression of VEGF.