EFFECTS OF VITAMIN D ON GLUCOSE AND LIPID METABOLISM, ENDOCRINE AND OVARIAN MORPHOLOGY AND FUNCTION IN RATS WITH POLYCYSTIC OVARY SYNDROME

Haiyan Cheng¹, Juanzhi Hao², Yanjie Song¹, Wenhua Zhang^1*

¹Department of Gynecology, The Affiliated Central Hospital of Qingdao University, Qingdao, 266042, China

²Department of Disease Control, The Affiliated Central Hospital of Qingdao University, Qingdao, 266042, China

Objective: This study aims to analyze the effects of vitamin D on glucose and lipid metabolism, endocrine and ovarian morphology, and function in rats with polycystic ovary syndrome. 

Methods: Forty-five clean grade healthy female SD rats were randomly divided into control, model, and vitamin D groups, with 15 rats in each. The rat model of polycystic ovary syndrome was established. Vitamin D rats were given 400 IU/kg/D vitamin D drops by gavage. The estrous cycle days, glucose and lipid metabolism indexes, endocrine indexes, vitamin D, and anti-Mullerian hormone (AMH) levels of rats in each group were compared. 

Results: The levels of FBS, TG, TC, LDL, fins, IRI, t, LH, and AMH were significantly increased, the estrous period was significantly shortened, and the levels of HDL, FSH, E2, P, and vitamin D were significantly decreased (P<0.05). Compared with the model group, the levels of FBS, TG, TC, LDL, fins, IRI, t, LH, and AMH in the vitamin D group were significantly decreased, the estrous period was significantly prolonged, and the levels of HDL, FSH, E2, P and vitamin D were significantly increased (P<0.05). In the control group, the development of follicles was normal, oocytes were clear, and multiple corpus luteum could be seen. In the model group, the ovarian follicles showed cystic expansion, the number of granular cells decreased, the follicular membrane thickened, and the number of corpus luteum decreased significantly. Compared with the model group, the number of follicles and corpus luteum in the vitamin D group decreased. 

Conclusion: Vitamin D can dramatically improve the estrous cycle, regulate glucose and lipid metabolism and endocrine levels, improve insulin resistance, and promote the recovery of ovarian morphology and function.