MIR-486-5P ENHANCES THE SENSITIVITY OF HUMAN NEUROBLASTOMA SH-SY5Y CELLS TO CISPLATIN BY DOWNREGULATING PIM1

Hang Sun^{1, 3, #}, Xiao Wang^{1, #}, Xiaoning Zhang^{1, 2}, Zhen Yue¹, Yanan Yang¹, Youjie Li^{1, *}, Yunxiao Sun^{3, *}

¹Key Laboratory of Tumor Molecular Biology in Binzhou Medical University, Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong 264003, P.R. China - ²Laboratory of Binzhou maternal and Child Health Hospital, Binzhou, Shandong 256600, P.R. China - ³Department of Pediatrics, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong 264100, P.R. China

Objective: As an antitumor drug, cisplatin plays a huge role in the treatment of various types of cancer. In this study, we observed the mechanism of cisplatin in the treatment of human neuroblastoma SH-SY5Y cells. 

Methods: This experiment uses reverse transcription-polymerase chain reaction (RT-PCR) technology, western blot analysis, and flow cytometry to detect the expression of miR-486-5p and PIM1 after cisplatin acts on SH-SY5Y cells, and predict the binding site of miR-486-5p and PIM1 using the prediction website.

Results: Reverse transcription-polymerase chain reaction (RT-PCR) results showed that cisplatin can promote the expression of miR-486-5p and found that miR-486-5p can inhibit cell proliferation. In addition, we transfect miR-486-5p into cells, and the results showed that PIM1 expression in the cells transfected with miR-486-5p was significantly lower than that in the control group. We combined cisplatin and miR-486-5p on SH-SY5Y cells and found that cell proliferation decreased, apoptosis increased, and PIM1 expression decreased compared with cisplatin or miR-486-5p alone. 

Conclusion: According to the present evidence, miR-486-5p can enhance the sensitivity of human neuroblastoma SH-SY5Y cells to cisplatin by downregulating PIM1. The findings of this study can provide a basis for drug design or new targets for cancer treatment.