IMPACTS OF CYP7A1 AND SLCO1B1 POLYMORPHISMS ON SERUM LIPID LEVELS IN HEALTHY TURKISH POPULATION

Zuhal Uckun Sahinogullari^{1, *}, Necmiye Canacankatan², Mehmet Canacankatan³, Cem Yalaza⁴

¹Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Mersin University, Mersin, Turkey - ²Depatment of Biochemistry, Faculty of Pharmacy, Mersin University, Mersin, Turkey - ³Mersin City Training and Research Hospital, Mersin, Turkey - ⁴Department of Medical Services and Techniques, Toros University, Vocational School, Mersin, Turkey

Introduction: Serum lipid levels can be affected by certain genes. CYP7A1 and SLCO1B1 genes may have a part in lipid metabolism. The distinctions in CYP7A1 and SLCO1B1 genotypes may be related to serum levels of lipid. The goals of this pilot study were to detect the frequencies of CYP7A1 g.-203A>C (rs3808607) and SLCO1B1 c.388 A>G (rs2306283) polymorphisms in a healthy Turkish population and to explore the relationship between these polymorphisms and serum levels of lipid. 

Materials and methods: Genomic DNA was obtained from 120 healthy Turkish participants, who fasted for 10-12 hours. CYP7A1 and SLCO1B1 polymorphisms were detected based on polymerase chain reaction-restriction fragment length polymorphism techniques. The serum lipid levels were determined by standard methods. 

Results: The frequencies were determined as 57.9% A allele and 42.1% C allele for CYP7A1 gene, 56.2% A allele and 43.8% G allele for SLCO1B1 gene. The serum lipid levels were affected by gender and also the gene-by-gender interaction for CYP7A1 genotypes, but this was not observed for SLCO1B1 genotypes.

Conclusion: This study is the first to document the frequencies of CYP7A1 g.-203A>C in the Turkish population and to reveal the relationship between CYP7A1 g.-203A>C or SLCO1B1 c.388 A>G polymorphism and serum levels of lipid.