EXPRESSION OF MIR-200A IN MENINGIOMA AND ITS RELATIONSHIP WITH ANGIOGENESIS

Lifang Si^*, Mengyun Li, Jian Li, 

College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, Henan Province, China

Objective: To investigate the expression of microrna-miR-200a (miR-200a) in meningioma and its relationship with angiogenesis. 

Methods: 55 cases of grade III malignant meningiomas diagnosed in our hospital from February 2017 to April 201 were selected for primary cell culture, and the vasculogenic mimicry (VM) model of meningioma cells was constructed. After successful modeling, the miR-200a low expression group (miR-200a - group) and miR-200a high expression group (miR-200a + group) were established by lentivirus transfection, and the negative control group miR-200a + was set up respectively, that is, with an NC group and a miR-200a + NC group. The expression levels of EMT related proteins (twist, E-cadherin), ve cadherin, Wnt and β-catenin, the number of penetrating cells, the ability of angiogenesis and the migration distance at 0 h, 6 h, 12 h and 24 h were compared. 

Results: The expression levels of twist and VE-cadherin protein in the miR-200a - group were significantly higher than those measured in the miR-200a + group, and the expression level of E-cadherin was significantly lower than that measured in the miR-200a + group (P<0.05). There was no significant difference in the expression of twist, VE-cadherin and E-cadherin between the miR-200a - group and the miR-200a + group (P>0.05). At 12 h and 24 h, the migration distance of the miR-200a - group was significantly higher than that of the miR-200a + group. At 12 h and 24 h, the migration distance of the miR-200a + group was significantly lower than that of the miR-200a + NC group. There was no significant difference between the miR-200a + NC group and the miR-200a-nc group (P>0.05). The number of transmembrane cells in the miR-200a - group was significantly higher than that measured in the miR-200a + group. There was no significant difference between the miR-200a + group and the miR-200a + NC group (P>0.05). The ability of angiogenesis in the miR-200a - group was significantly stronger than that measured in the miR-200a + group. The expression of Wnt and β-Catenin protein in the miR-200a - group was significantly higher than that in the miR-200a + group (P<0.05). The expression levels of Wnt and β-catenin in miR-200a + group were significantly lower than those measured in the miR-200a + NC group (P<0.05). There was no significant difference in the expression of Wnt and β-catenin between the miR-200a + NC group and the miR-200a-nc group (P>0.05). 

Conclusion: The decreased expression of miR-200a in meningiomas can promote the migration, invasion and angiogenesis of meningioma cells, which may be achieved by activating the Wnt / β-catenin signaling pathway.