EFFECT OF SHRNA INTERFERING B7-H3 GENE EXPRESSION ON THE PROLIFERATION AND INVASION OF BLADDER UROTHELIAL CARCINOMA T24 CELLS

Bin Zheng, Qiquan Wu, Ren Wang, Gang Wang, Yu Ren, Guobin Weng^*

Ningbo Yinzhou District Second Hospital, Ningbo, PR China

Objective: To investigate the effect of shRNA interference on the proliferation and invasion of bladder urothelial carcinoma T24 cells.

Methods: Thirty cases of bladder urothelial cell carcinoma and adjacent normal tissues were randomly selected from March 2019 to February 2020 in our hospital. The expression of B7-H3 in bladder urothelial carcinoma and adjacent normal tissues was detected by immunohistochemistry. T24 bladder cancer cells were cultured and infected with lentivirus. After culturing, B7-H3 low expression group and control group cells were obtained. Each group of cells was set with 4 multiple holes. The expression levels of B7-H3 and matrix metalloproteinase-2 (MMP-2) were measured via real-time fluorescent quantitative PCR and Western blotting. MTT assay methods were used to determine the proliferation of cells in each group, and a transwell invasion assay was used to determine the invasion ability of the two groups.

Results: The expression of B7-H3 in bladder urothelial carcinoma was significantly higher than that measured in the adjacent normal tissues (P>0.05). There was no significant difference in cell proliferation between the two groups at 0 hours (P>0.05). From 24 hours, as compared to the control group, the proliferation ability of the B7-H3 low expression group was significantly decreased (P<0.01). Likewise, the invasion ability of the B7-H3 low expression group was significantly decreased (P<0.01) as compared to the control group. Further, as compared to the control group, the expression levels of B7-H3 and MMP-2 in the B7-H3 low expression group were significantly decreased (P<0.01). 

Conclusion: The interference of shRNA in B7-H3 gene expression can significantly inhibit the proliferation and invasion of bladder urothelial carcinoma T24 cells, implying an important target for the diagnosis and treatment of bladder cancer.