CLINICAL VALUE OF COMBINED DETECTION OF MIR-490-5P AND DICER1 IN THE DIAGNOSIS OF RECTAL CANCER

Zhiqiang Xuan^* 

Department of The First Ward of Gastrointestinal Surgery, Dalian Municipal Central Hospital, Dalian 116000, Liaoning Province, China

Objective: To analyze the clinical value of combined detection of mir-490-5p and Dicer1 in the diagnosis of rectal cancer. 

Methods: Seventy-eight patients with colorectal cancer admitted to our hospital from January 2019 to January 2020 were recruited, and 40 healthy people in our hospital for physical examination were selected as the control group. The levels of mir-490-5p and Dicer1 were detected by reverse transcription and real-time quantitative PCR. The levels of mir-490-5p and Dicer1 in the serum of the two groups were compared. The levels of mir-490-5p and Dicer1 in CRC patients with different clinical symptoms were compared. A ROC curve was used to analyze the diagnostic efficacy of mir-490-5p and Dicer1 in rectal cancer. A multivariate Cox risk ratio model was used to analyze the prognostic risk factors of CRC patients. 

Results: The expression levels of mir-490-5p and Dicer1 in the observation group were significantly lower than those in the control group (P<0.05). There was no significant correlation between serum mir-490-5p expression and age, gender, tumor location, lymph node metastasis and TNM stage (P>0.05); serum Dicer1 expression level had no significant correlation with age, gender and tumor location (P>0.05), but had a certain relationship with lymph node metastasis and TNM stage (P<0.05). A Pearson linear correlation analysis showed that mir-490-5p was positively correlated with Dicer1 (r = 0.756, P<0.01). ROC curve analysis showed that the AUC of mir-490-5p in the diagnosis of CRC was 0.761; the sensitivity was 78.62%; the specificity was 79.31%; the AUC of Dicer1 in the diagnosis of CRC was 0.701; the sensitivity was 78.61%, and the specificity was 71.04%. The AUC of the combined diagnosis of CRC was 0.815; the sensitivity was 83.14%; and the specificity was 82.94%. Multivariate Cox proportional hazards model analysis showed that lymph node metastasis, TNM stage and Dicer1 were risk factors for the prognosis of CRC patients (P<0.05 or<0.01).

Conclusion: The serum levels of mir-490-5p and Dicer1 in patients with rectal cancer are significantly lower than those in patients with rectal cancer, and they are significantly positively correlated. These factors have a certain value in the diagnosis of rectal cancer and the use of both in combination has the highest value and can be widely used in clinical practice.