THE EFFECT AND MECHANISM OF DAGLIEJING ON RENAL FIBROSIS IN RATS WITH DIABETIC NEPHROPATHY

Feng Tan, Jiaying Wang, Binxiao Ge, Chenji Wu, Yuan Feng^* 

Department of Nephrology, Tongchuan People's Hospital, Tongchuan 727000, PR China

To investigate the effect of daggliptin on renal fibrosis in rats with diabetic nephropathy and to analyse its target and molecular mechanism. For this study, 60 rats were randomly and equally divided into two groups. The control group received daily injections of saline, while the observation group received daily injections of 3 mg/kg dagglizine. After 12 weeks, the kidneys were removed, and the kidney weight/body weight ratios were compared. The concentrations of urine protein, urine creatine (UCR), blood urea nitrogen (BUN), malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured by the kit. The concentrations of collagen Ⅳ, fibronectin, E-cadherin, α-smooth muscle actin, α-SMA, N-cadherin, Smad2, Smad3, phosphorylated Smad2 and p-Smad3 were detected by ELISA and transforming growth factor β1 (TGF-β1). Dagglitazine can significantly reduce renal fibrosis in rats with diabetic nephropathy and can provide important clinical application data and evidence to support the effective treatment of nephropathy and renal fibrosis.