MIR-182-5P PROMOTES PROLIFERATION AND METASTASIS OF GASTRIC CANCER CELLS BY TARGETING CEBPA/PPARG/ATG7 SIGNAL AXIS

Hongtao Wang^{1, *}, Haifeng Li², Yucen Liang¹

¹Department of General Surgery, Wuwei People's Hospital, Wuwei 733000, Gansu Province, China - ²Department of Outpatient, Wuwei People's Hospital, Wuwei 733000, Gansu Province, China

Objective: To analyze the effect of mir-182-5p on the proliferation and metastasis of gastric cancer cells and the mechanism of this effect. 

Methods: SGC-7901 cells were divided into a control group, a mir-182-5p overexpression group, and a mir-182-5p inhibition group. We added a template-free control to the control group, a miRNA agonist to the mir-182-5p overexpression group, and a miRNA antagonist to the mir-182-5p inhibition group. We then used a CCK-8 cell proliferation test to detect cell proliferation, a scratch test to detect cell metastasis, fluorescent labeling technology to detect autophagy, and the Western bolt method to detect the expression of the CEBPA/PPARG/Atg7 signal axis and autophagy-related proteins. 

Results: The cell proliferation of each group increased over time. After 48 hours and 72 hours, the cell proliferation, metastasis, and autophagy of the mir-182-5p overexpression group were significantly higher than that of the control group, whereas the cell proliferation, metastasis, and autophagy of the mir-182-5p inhibition group were significantly lower than that of the control group (P<.05). Similarly, the levels of Beclin1, lc3b - Ⅱ/I, and Atg7 protein in the mir-182-5p overexpression group were significantly higher than those in the control group, while such levels in the mir-182-5p inhibition group were significantly lower than those in the control group (P<.05). Finally, the levels of C/EBP α and PPAR γ protein were significantly lower in the mir-182-5p overexpression group and significantly higher in the mir-182-5p inhibition group, as compared to the control group (P<.05). 

Conclusion: The overexpression of mir-182-5p can promote the proliferation, metastasis, and autophagy of gastric cancer cells, and its mechanism may be related to its regulation of CEBPA/PPARG/Atg7 signal axis-related proteins.