STUDY ON THE EFFECT OF COMPOUND “ZICAO” TEMPERATURE-SENSITIVE GEL ON SKIN WOUND HEALING OF NEW ZEALAND RABBITS

Yunjie Wu, Ke Xu, Xiaoxia Liang^*

Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, PR China

In this study, a temperature-sensitive gel made by our lab was used to evaluate the effect of compound comrador on skin wound healing in New Zealand rabbits. First of all, New Zealand rabbit skin excision injury model was established and four will be randomly divided into treatment group (normal saline negative control group, yunnan baiyao group, the blank group, the compound radix arnebiae seu lithospermi matrix group win min gel), to deal with New Zealand rabbit was detected after 14 d of wound healing rate and hydroxyproline content, HE dyeing observation was carried out on the wound tissue fibroblasts epithelization degree,Masson staining was performed to observe the fibers and inflammatory factors in the wound tissue. The results showed that after 14 days of treatment, the wound healing rate of New Zealand rabbits in compound comrador temperature-sensitive gel group was significantly higher than that in other treatment groups, and the wound healing rate was 92.4%. In addition, the histological analysis of wound tissue of New Zealand rabbits treated with compound comradina thermosensitive gel also showed that the degree of epithelialization was increased and the proliferation of fibroblasts was enhanced. Through the evaluation of its mechanism, the compound thermal-sensitive gel could also increase the content of hydroxyproline. The total hydroxyproline content of wound tissue increased to 3.51μg·mg-1 after the treatment of compound thermal-sensitive gel, which further confirmed that it could protect cells from oxidative damage, thus accelerating wound healing and reducing wound healing time. In conclusion, the topical application of compound thermosensitive gel made by ourselves in this study can promote wound healing in New Zealand rabbits, and provide a new and better choice for wound healing.