Metabonomics of Brucellosis and Brucellosis Spondylitis in Xinjiang

Zhenfeng Liu¹, Jun Wang¹, Wei Hu^2,*, Shujun Zhang¹ 

¹Wuxi Hand Surgery Hospital, Wuxi 214000, Jiangsu, China - ²Affiliated Hospital of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi 830000, Xinjiang, China

Introduction: Xinjiang is an area with a high incidence of brucellosis, a zoonotic and infectious allergic disease. Brucellosis often invades the spine and causes brucellosis spondylitis, which severely affects patient health. The purpose of this study was to identify brucellosis biomarkers and improve the diagnosis of brucellosis. 

Materials and methods: In this study, ultra-high-performance liquid chromatography (uHPLC) and high-resolution mass spectrometry (HRMS) were used to detect differences in metabonomics between patients with brucellosis and brucellosis spondylitis.

Results: The results showed that there were 24 differential metabolites between the brucellosis and control groups, including alanine 1, palmitic acid, serine 1, linoleic acid, α ketocopheric acid 1, cysteine, α tocopherol, 4 hydroxybenzoic acid, cycloleucine 1, monostearine, and fumaric acid. The differential metabolites mainly enriched the linoleic acid metabolism metabolic pathway. Moreover, we found that the biomarkers were closely related to the occurrence and development of the above two diseases. We also analyzed the changes and roles of related metabolic markers in different stages of brucellosis and brucellosis spondyliti. 

Conclusion: This study provides new ideas and a scientific basis for studying the pathogenesis, prevention, and treatment of the above two diseases.