KOUMISS THERAPY CAN IMPROVE THE INTESTINAL FLORA IMBALANCE IN PATIENTS WITH HYPERLIPIDEMIA IN A SHORT TIME

Siqin Bateer¹, A Nan Da¹, Uugan Jargal², Su Niri¹, Bao Leri¹, Aodeng Bilige¹, Ao Qi¹, Zhang Xiaoxiao¹, Tegexibaiyi Wang^{2, *}

¹Xilin Gol League Mongolian Medical Hospital. No.187 Nadamu Road, Xilinhot, Inner Mongolia Autonomous Region, China - ²Pharmacy laboratory, Inner Mongolia International Mongolian hospital, NO. 83 daxuedong Road, Hohhot 010065, China

Objective: To investigate the changes in the structure and function of intestinal flora in patients with hyperlipidemia after the intervention of Koumiss. 

Methods: the fecal samples of hyperlipidemia patients were collected at 0, 7, and 14 days after the treatment of Koumiss. The V3 + V4 region of 16S rRNA gene was amplified and analyzed by high-throughput sequencing α Diversity β Diversity analysis, species composition analysis at the phylum level, family level, and genus level, significance analysis of group differences, and functional gene prediction analysis. 

Results: the abundance and diversity of intestinal flora in hyperlipidemia patients increased after 14 days of treatment with koumiss. The ace index and Chao index of the 0-day group were significantly lower than those of the 7-day group and 14-day group (P<0.01); The ace index and Chao index of the 14-day group were significantly higher than those of the 7-day group (P<0.01); At the phylum level, the relative abundance ratio of Firmicutes to Bacteroidetes (Firmicutes / Bacteroidetes) increased from 1.36 (units) to 3.36 (units) before drinking. At the family level, the number of Trichospirillum increased significantly after 14 days of treatment. At the genus level, agathobacter, subdoligranulum, Enterobacter, and Bacteroides accounted for the highest proportion. 

Conclusion: the results of this study show that koumiss therapy can affect the structure of intestinal flora in patients with hyperlipidemia in a short time, regulate the balance of intestinal flora, improve intestinal immune function, and reduce high cholesterol and high triglyceride, which can be widely used in the clinic.