Genetic polymorphism and genotype analysis of ApoE4 and CR4 in patients with Alzheimer’s disease

Xin-Ran Wu¹, Ling Gou¹, Ru-Jiao Wang², Feng-Qin Sun³, Wei Wang⁴, Wei Huang^3* 

¹Department of Science and Education, The Third People’s Hospital of Yunnan Province, The Second Affiliated Hospital of Dali University, Kunming 650011, China - ²Clinical College, Dali University, Dali 671000, Yunnan, China - ³Department of internal neurology, The Third People’s Hospital of Yunnan Province, The Second Affiliated Hospital of Dali University, Kunming 650011, China - ⁴Department of neurorehabilitation, Psychiatric Hospital Of Yunnan Province, Kunming 650224, China

Objective: This study aimed to determine whether it is feasible for the polymorphism of ApoE4, EApoE and CR4 complement receptor 1 (CRl) loci genotypes in Alzheimer’s disease (AD) patients to serve as an effective and susceptible diagnosis index for screening clinical AD. 

Methods: The PCR technique was employed to conduct the genotype sequencing of Apo, EApoE and CR1 genes in 50 AD patients and 50 healthy controls. Simultaneously, SNP analysis was also performed.

Results: There was a significant difference among the three loci (rs381836l, rs6656401 and rs7412). Hence, this can be separately considered as the susceptible locus for AD screening and primary diagnosis. However, there was no marked difference for locus rs429358 relative to the other three. Therefore, this suggests that rs429358 should not be considered as a susceptible gene locus for AD screening and primary diagnosis.

Conclusion: There is a difference in rs7412, which can be used as a reference site for judgment.