CHANGES OF MIR-144-3P AND MIR-337-3P IN SERUM OF CERVICAL CARCINOMA PATIENTS AND ANALYSIS OF DIAGNOSIS AND PROGNOSIS

Donglei Li^1#, Xinmiao Ge^2#, Peng Liu^2*

¹Department of Oncology, Baoding Second Central Hospital, Baoding 072750, China - ²Department of Obstetrics and Gynecology, Baoding Second Central Hospital, Baoding 072750, China

Objective: To analyze the expression changes of miR-144-3p and miR-337-3p in serum of cervical carcinoma (CC) patients and their diagnosis and prognosis. 

Methods: Altogether 72 cases of CC patients and 63 cases of health people were selected as the research participants,with CC patients as the research group(RG) and health people as the control group(CG). miR-144-3p and miR-337-3p in serum of the CG and the RG was detected, the diagnostic value of miR-144-3p and miR-337-3p to CC was analyzed, the expression changes of miR-144-3p and miR-337-3p before and after treatment in the RG were observed, and the relationship of the expressions of miR-144-3p and miR-337-3p with the clinicopathological characteristics of CC was analyzed respectively. Human cervical carcinoma cell C33A and normal cervical epithelial cell H8 were purchased for biological behavior analysis, and the influence of miR-144-3p and miR-337-3p on the prognosis of CC patients after treatment was observed. 

Results: The expression of miR-144-3p and miR-337-3p in the serum of patients in the RG was lower than that in the CG (P<0.05). miR-144-3p and miR-337-3p had good diagnostic value for CC. The expression levels of miR-144-3p and miR-337-3p in the RG increased evidently after treatment (P<0.05). miR-144-3p and miR-337-3p were closely related to cell differentiation, clinical stage and lymph node metastasis of CC patients (P<0.05). The cell proliferation and invasion ability of over-expression of miR-144-3p and miR-337-3p were lower than those of the other two groups, while the apoptosis rate was higher than that of the other two groups (P<0.05). The prognosis of high miR-144-3p group was better than that of low miR-144-3p group (P=0.020), and the prognosis of high miR-337-3p group was better than that of low miR-337-3p group (P=0.041). 

Conclusion: miR-144-3p and miR-337-3p are closely related to cervical carcinoma and may be the key to the diagnosis and treatment of cervical carcinoma in the future.