The fat pat tissue expresses a pathogenic profile of inflammatory mediatories and 4-HNE in patients with osteoarthritis

Bulent Tanriverdi^1*, Gamze Tanriverdi², Ersin Ercin¹, Fatma Kaya Dagistanli³, Halil Nadir Ones¹, Erdem Edipoglu¹, Sibel Demirci Delipinar⁴, Basak Isildar², Mustafa Gokhan Bilgili¹

¹Department of Orthopaedics, University of Health Science, Bakirkoy Dr. Sadi Konuk Training and Research Hospital, Istanbul, Turkey - ²Department of Histology and Embryology, Cerrahpasa Medical Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey - ³Department of Medical Biology, Cerrahpasa Medical Faculty, Istanbul University- Cerrahpasa, Istanbul, Turkey - ⁴Department of Histology and Embryology, Medical Faculty, Biruni University, Istanbul, Turkey

Introduction: In this study we aimed to estimate if there is a releation between some inflammatory or adipocyte-derived cytokines such as IL-1 beta, IL-6, TNF-alfa, VEGF, 4-HNE, macrophages, mast cells and osteoarthirites. Especially 4-HNE which associated with obesity is a new marker for estimating the chondral injuries and there is a poor evidence of it’s effect on IFP and osteoarthritis.

Methods: We obtained tissues after ethical approving and designed our studying groups as one osteoarthritis (n=15) and one non-osteoarthritis patients group (n=12) and then applied histological procedure for estimating the IFP tissues. We stained using IL-1beta, IL-6, TNF-alfa, VEGF, 4-HNE, CD68 (for macrophage marker) antibodies by immunohistochemical technique and toluidine blue for the mast cells, examined slides microscopically and evaluated all of the tissues according to the staining intensity by using HSCORE.

Results: As a result, we detected high expression levels of inflammatory mediators especially IL-6, IL-1beta, VEGF and 4-HNE in the osteoarthritis group (OAG) than the non-osteoarthritis group (NOAG) and the numbers of macrophages and mast cells are higher in the OAG, too. Also, the macrophages have distinctly different morphologies in the OAG and they were bigger and had crown like structures compare to the NOAG.

Conclusions : In conclusion, these data indicate that IFP-derived inflamatory mediators could contribute to pathophysiological processes in the osteoarthritis of the knee joint and there is a necessity for future studies in this area.