THE EFFECT OF SCUTELLARIN ON APOPTOSIS AND THE EXPRESSION OF BCL-2 AND BAX IN GLIOMA C6 CELLS

Lv Peng^{1, 2}, Xue Baiji^{2, *}, Wang Yongsheng^{1, *}

¹School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China- ²School of Basic Medical Sciences, Baicheng Medical College, Baicheng 137000, China

Objective: To explore the inhibitory effect of Scutellarin(SCU) on prolifenation of rat glioma C6 cells, and to clarify its mechanism. 

Methods: The C6 cells were cultured and divided into blank control group and 5, 10, 20, 40, 80 mg·L-1 SCU groups, the surival rates of C6 cells in various were examined by MTT assay; the morphology of C6 cells in various groups were observed by inverted microscope; the apoptotic rates of C6 cells were examined by Annexin V/PI staining; the expression levels of bax and bcl-2 proteins in the C6 cells in various group were detected by cell immunohistochemistry.

Results: The MTT results showed that compared with blank control group, the survival retes of C6 in 10, 20, 40 and 80 mg·L-1SCU at 48h were decreased (P﹤0.01);And the survival retes of C6 in 20, 40 and 80 mg·L-1SCU at 72h were decreased (P﹤0.01). The group, the C6 cells in SCU group was significantly decreased, some cells became inverted microscope observation results showed that compared with blank control round and volume reduced, and cell contour was not clear. The results of Annexin V/PI staining showed that the late and total apoptotic rates of C6 cells in 80 mg·L-1 SCU group were increased compared with blank control group (P﹤0.05). The cell immunohistochemistry results showed that compared with blank control group, the  expression levels of bax protein in the C6 cells in 80 mg·L-1 SCU group were increased, and the bcl-2 protein were decreased; Compared with blank control group, the ratio of bax/bcl-2 in the C6 cells in SCU group was significantly increased (P﹤0.05). 

Conclusion: SCU induce the apoptosis and suppress the tumor growth by upregulating the bax/bcl-2 in the C6 cells.