Lin Chu1, #, *, Dezhan Li2, #, Xuefen Fei3, Wenming Feng4, Hongxing Zhao4
1Department of Thoracic surgery, Affiliated RenHe Hospital of China Three Gorges University, Yichang, Hubei, 443000, PR China -
2Department of Anesthesiology, Jingzhou Central Hospital, The Second Clinical Medical College, Yangtze University, Jingzhou, Hubei, 434020, P R China - 3Department of Respiration, The Linghu People’s Hospital of Nanxun District Huzhou, Huzhou 313018, Zhejiang, PR China - 4Department of Surgery, The First People’s Hospital of Huzhou (The First Affiliated Hospital of Huzhou University), Huzhou 313000, Zhejiang, PR China
Objective: To investigate the effect of TIGAR knockout on the apoptosis of A549 cells and expression of epitheli-al/mesenchymal markers in doxorubicin (DOX)-resistant non-small cell lung cancer (NSCLC).
Methods: A DOX-resistant A549 cell line (A549/DOX) was established in this study, and the gene was knocked out through TIGAR-targeted siRNA reverse transfection. The TIGAR protein, cell viability, colony survival, ROS, NADPH and expression levels of apoptosis-related markers and epitheli-al/mesenchymal markers of the control group and siRNA transfection group were compared.
Results: After DOX treatment, the cell viability of A549/DOX cell line was significantly higher than that of the original A549 cell line (P<0.05). The TIGAR protein, cell viability, colony survival, levels of NADPH, Bcl-2, PARP and vimentin in the siTIGAR transfection group were significantly lower than those in the control group (P<0.05). The levels of ROS, Bax, Caspase-3, Caspase-9, PARP degradation products and E-caderin in the siTIGAR transfection group were significantly higher than those in the control group (P<0.05).
Conclusion: TIGAR knockout can inhibit the EMT of DOX-resistant NSCLC and be used as a potential therapeutic target for patients with chemotherapy resistance.
TIGAR, lung cancer, targeted therapy, apoptosis, epithelial-mesenchymal transition.