Authors

Honggui Ma1, Liansheng Zhang2, Facai Zhang3, Fanghao Sun1, Jianquan Hou4, *


Departments

1Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou, PR China - 2Department of Urology, Soochow University Affiliated Wuxi Ninth Hospital, Wuxi, PR China - 3Department of Urology, The Affiliated Hospital of Guizhou Medical University, Guiyang, PR China - 4The First Affiliated Hospital of Soochow University, Suzhou, PR China

Abstract

Objective: To investigate the correlation between the expression levels of human cartilage glycoprotein 39 (YKL-40) and cell migration and invasion abilities in human prostate cancer DU145 and PC3 cell lines.

Methods: Prostate cancer tissue and paracancerous tissue were collected from 58 patients in our hospital from May 2016 to January 2018. The expression of YKL-40 in prostate cancer tissues and paracancerous tissues was detected by immunohistochemistry. Human prostate cancer DU145 and PC3 cell lines were collected, and the invasion and migration abilities and YKL-40 mRNA expression of DU145 and PC3 cell lines were detected by Transwell chamber and Western blot methods, respectively. PC3 cells were randomly divided into a PC3-WT group (wild-type PC3 cells), a PC3-control group (empty vector), a PC3-YKL-40 group (up-regulation of YKL-40 expression), and a PC3-YKL-40-KD group (down-regulation YKL-40 expression) DU145 cells were randomly divided into a DU-145-WT group (wild-type DU-145 cells), a DU-145-control group (empty vector), a DU-145-KD-YKL-40 group (up-regulation of YKL-40 expression), and a DU-145-KD group (down-regulation of YKL-40 expression). Cell invasion and migration abilities of each group were analysed and compared.

Results: The high expression rate of YKL-40 was 72.41% (42/58) in prostate cancer tissue and 10.34% (6/58) in paracancerous tissues, with statistical significance between the groups (p<.05). Compared with PC3 cells, DU145 cell invasion and migration abilities, YKL-40 protein and mRNA expression levels were significantly increased (p<.05). The cell invasion and migration abilities of the PC3-YKL-40 group were significantly higher than that of the PC3-WT group, and the cell invasion and migration abilities of the PC3-YKL-40 group were significantly higher than that of the PC3-YKL-40-KD group (p < .05). Compared with the PC3-control group and the PC3-YKL-40-KD group, the cell invasion and migration abilities of the PC3-WT group had no significant difference (p>.05). The cell invasion and migration abilities of the DU-145-KD group were significantly lower than those of the DU-145-WT group (p<.05). The Cell invasion and migration abilities of the DU-145-KD group were significantly lower than those of the DU-145-KD-YKL-40 group (p<.05). Compared with DU-145-control group and DU-145-KD-YKL-40 group, the cell invasion and migration abilities of the DU-145-WT group had no significant difference (p>.05).

Conclusion: The expression of YKL-40 is significantly increased in prostate cancer, and the expression level of YKL-40 in prostate cancer DU145 and PC3 cell lines is positively correlated with cell migration and invasion abilities.

Keywords

Prostate cancer, DU145 cells, PC3 cells, YKL-40, migration, invasion.

DOI:

10.19193/0393-6384_2021_2_152