INHIBITORY EFFECT OF CELECOXIB ON PROLIFERATION, APOPTOSIS AND CELL CYCLE ARREST OF LARYNGEAL CARCINOMA CELLS THROUGH COX-2 RELATED PATHWAY

Hui Chen¹, Mudunov Ali^{2, *}, Azizyan Ruben², Dimitry Stelmakh², Maksim Pak²

¹I.M. Sechenov First Moscow State Medical University N.N. Blokhin Nathional Medical Research Center of the Oncology of the Ministry of Health of the Russian Federation, Anyang, PR China - ²N.N. Blokhin Nathional Medical Research Center of the Oncology of the Ministry of Health of the Russian Federation, Moscow, Russia

Objective: To investigate the effect and mechanism of celecoxib on inhibiting proliferation, inducing apoptosis and cell cycle arrest of laryngeal carcinoma tumour cells through cyclooxygenase-2 (COX-2) related pathway. 

Methods: Hep-2 cells were treated with different concentrations of celecoxib medium. Namely, 10 μm, 30 μm, 50 μm, 70 μm and 100 μm celecoxib groups and a blank control group were cultured for 12 h, 24 h and 48 h. The effects of celecoxib on cell activity, apoptosis, cell cycle and related proteins (COX-2, Bcl-2, p16^INK4a, p27^KIP, p 21^waf/cip1) were observed. 

Results: Compared with the control group at 12 h, 24 h and 48 h, the cell inhibition rate of celecoxib group (10 μm, 30 μm, 50 μm, 70 μm, 100 μm) was significantly higher than that of the control group (P<0.05). Compared with the control group, the apoptosis rate of celecoxib group (10 μm, 30 μm, 50 μm, 70 μm, 100 μm) increased significantly (P<0.05) in a dose-dependent manner. Compared with the control group, the proportion of G0/G1 in celecoxib group (10 μm, 30 μm, 50 μm, 70 μm, 100 μm) increased significantly, while the proportion of S decreased significantly in a dose-dependent manner. Compared with the control group, the expression of COX-2, Bcl-2 protein in celecoxib group (30 μm, 50 μm, 70 μm, 100 μm) decreased significantly, while the expression of p27KIP protein in p16INK4A group was significantly higher than that in the control group (P<0.05). There was no significant difference in p21waf/cip1 protein expression between the celecoxib group and the control group (P>0.05). 

Conclusion: The inhibitory effect of celecoxib on the proliferation, apoptosis and cell cycle arrest of laryngeal carcinoma cells may be achieved through a COX-2 related pathway.