EFFECTS OF GUANXINNING TABLETS ON THE AUTONOMIC NERVE REGULATION FUNCTION OF MI/ RI RATS BY ANTIOXIDATION AND INHIBITION OF INFLAMMATORY RESPONSE

Liang Xu¹, Ling Zhou², Jian Dai¹, Yongbo Lin^{3, *}

¹Department of ICU, Wuhan Wuchang Hospital,Wuchang Hospital Affiliated to Wuhan University of Science and Technology, Wuhan, PR China - ²Department of ICU, People's Hospital of Dongxihu District, Wuhan, PR China - ³Department of Cardiology, People's Hospital of Dongxihu District, Wuhan, PR China

Objective: To investigate the effects of Guanxinning tablets on the autonomic nervous system regulation of myocardial ischemia/reperfusion (MI/RI) rats through anti-oxidation and inhibition of inflammatory response. 

Methods: One hundred (100) clean male Wistar rats were selected and randomly divided into a sham operation group, MI/RI group, and Guanxinning tablet low-dose group, middle-dose group, and high-dose group, using the random number table method. There were 20 rats in each group. Rats in the sham operation group and MI/RI group were injected with distilled water intraperitoneally, and those in the low, medium and high dose groups of Guanxinning were given different doses of Guanxinning tablets –60 mg/kg, 120 mg/kg, 180 mg/kg, respectively. Each group was continuously administered for 5 days. The MI/RI operation model was established, and the sham operation group was not treated. Suture lines in the MI/RI group and Guanxinning groups were tightened after administration. The changes in left ventricular function [left ventricular end-systolic pressure (LVESP) and left ventricular end-diastolic pressure (LVEDP)] of rats in each group, oxidative stress indicators [malonaldehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px)], inflammatory factors [interleukin-10 (IL-10), interleukin-6 (IL-6), and tumour necrosis factor-α (TNF-α)], heart rate variability (HRV) (RR interval, total power, high frequency, low frequency, and low frequency/high frequency), and the pathological morphology of myocardial tissue in rats were observed. 

Results: In comparison to the sham operation group, the levels of LVESP, SOD, GSH-Px, and IL-10 in the MI/RI group were significantly reduced, and the levels of LVEDP, MDA, IL-6, and TNF-α were significantly increased (P<0.05). In comparison to the MI/RI group, the levels of LVESP, SOD, GSH-Px, and IL-10 in the low, medium, and high-dose Guanxinning groups were significantly increased, and the levels of LVEDP, MDA, IL-6, and TNF-α were significantly decreased (P<0.05); With an increase in the dose of Guanxinning tablets, the levels of LVESP, SOD, GSH-Px, and IL-10 increased significantly, and the levels of LVEDP, MDA, IL-6, and TNF-α decreased significantly (P<0.05). In comparison to the sham operation group, the RR interval, total power, and high frequency in the MI/RI group were significantly reduced, and the low frequency and low-frequency/high-frequency ratios were significantly increased (P<0.05). In comparison to the MI/RI group, the RR interval, total power, and high frequency of the Guanxinning groups were significantly increased in the medium- and high-dose groups, and the low frequency and low-frequency/high-frequency ratios were significantly reduced (P<0.05). There was no significant difference in HRV indicators between the RI group and the Guanxinning low-dose group (P>0.05). In the sham operation group, myocardial fibres were arranged neatly, the nuclei were normal, and there was no inflammatory cell infiltration in the cardiac muscle stroma. In the MI/RI group, the myocardial fibres were distributed in a wavy shape, in a disordered arrangement, and some myocardial fibres and cell nuclei were broken or ruptured. The Guanxinning low-dose group exhibited slight improvements in comparison to the MI/RI group; in the medium-dose group, myocardial fibres were arranged neatly, and there was a small amount of inflammatory cell infiltration into the cardiac muscle stroma; the high-dose group was very similar to the sham operation group. 

Conclusion: Guanxinning tablets can significantly improve the cardiac autonomic nervous regulation function of MI/RI rats through anti-oxidation and inhibition of inflammatory response.