CORRELATION OF SERUM 8-HYDROXYDEOXYGUANYLATE LEVEL WITH DISEASE SEVERITY AND OXIDATIVE STRESS IN OSAHS PATIENTS

Wei Liu¹, Yuanyuan Fang², Miao Luo², Kui Liu^{2, *}

¹Department of Pulmonary and Critical Care Medcine, Central Hospital of Wuhan, Tongji Medical College Huazhong University of Science & Technology, Wuhan, PR China - ²Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China

Objective: To study the correlation between serum 8-hydroxydeoxyguanylate levels, disease severity, and oxidative stress in patients with obstructive sleep apnea-hypopnea syndrome.

Methods: Sixty OSAHS patients – treated at our department of respiratory medicine from July 2018 to July 2019 – were selected for the study group. According to their sleep apnea-hypopnea index (AHI) and minimum blood oxygen saturation (SaO2min), OSAHS patients were divided into three groups: mild group (n=24), moderate group (n = 20), and severe group (n=16). Healthy people were collected in the hospitals’ physical examination centre as the control group (n=30). Five millilitres of fasting blood of all the subjects were taken and tested, and the serum 8-hydroxydeoxyguanylate (8-OHdG), brain hemoglobin (NGB), malondialdehyde (MDA), supertrophic disproportion Enzyme (SOD), and glutathione peroxidase (GSH-Px) levels were compared to investigate the relationship between serum 8-OHdG levels, disease severity, and oxidative stress in OSAHS patients.

Results: There were significant differences in 8-OHdG, NGB, and MDA levels in each group: the severe group>the moderate group>the mild group >the control group (P<0.05). There were also significant differences in SOD and GSH-Px levels: the severe group <the moderate group <the mild group <the control group (P<0.05). Through Pearson linear analysis, it was found that 8-OHdG was directly proportional to AHI and NGB (r=5.112, 3.251, 2.436, P<0.05), and inversely proportional to SaO₂min (r=–5.243, P<0.05). 8-OHdG was negatively correlated to SOD and GSH-Px (r=–6.221, –5.489, P<0.05), and was positively correlated to MDA (r=3.251, P <0.05).

Conclusion: The level of serum 8-OHdG in OSAHS patients is higher than that in healthy people, and it is positively correlated to the disease severity and oxidative stress.