CORRELATION BETWEEN SERUM MIRNA-206, MIRNA-155 LEVELS AND THYROID HORMONES IN PATIENTS WITH HYPERTHYROIDISM AND ITS PREDICTIVE VALUE OF RECURRENCE

Xiaowei Niu¹, Honghai Zhang², Fei Wang³, Shuqing Ji³, Sheng Wang⁴, Xun Wu^{5, *}

¹Department of Chinese Traditional Surgery, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, PR China - ²Department of Surgery, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, PR China - ³Department of Orthopedics, Beijing TCM Hospital Ping Gu Hospital, Beijing, PR China - ⁴Department of Surgery, Beijing TCM Hospital Ping Gu Hospital, Beijing, PR China - ⁵Department of Education, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, PR China

Objective: To study the correlation between serum miRNA-206, miRNA-155 levels and thyroid hormones in patients with hyperthyroidism and their importance in predicting the recurrence of hyperthyroidism. 

Methods: 110 patients with hyperthyroidism admitted to our hospital from July 2018 to July 2019 were selected as the observation group, and 50 healthy people who were examined in our physical examination centre in the same period were selected as the control group. The observation group was divided into the relapse group (n = 50) and the non-relapse group (n = 60) according to the prognosis of the patients. T3, T4, and TSH levels of hyperthyroidism in patients in each group were detected by electrochemiluminescence, and real-time reverse transcription polymerase was used the levels of miRNA-206 and miRNA-155 were detected by a chain reaction. To compare the levels of miRNA-206, miRNA-155, T3, T4, and TSH in each group, to study the correlation between serum miRNA-206, miRNA-155, and T3, T4, and TSH in patients with hyperthyroidism, and to explore the value of serum miRNA-206, miRNA-155 in predicting thyroid recurrence in patients with hyperthyroidism. 

Results: The serum miRNA-206 level of the observation group was significantly lower than that of the control group, and the miRNA-155 level was significantly higher than that of the control group (P<0.05). The serum TSH level of the observation group was significantly lower than that of the control group, and the T3 and T4 levels were significantly higher than that of the control group (P<0.05). MiRNA-206 was negatively correlated with T3, T4 and miRNA-155 (r-0.589, - 0.632, - 0.695, P<0.05), positively correlated with TSH (r = 0.632, P<0.05); miRNA-155 was positively correlated with T3 and T4 (r = 0.549, 0.531, P<0.05), positively correlated with TSH and miRNA-206 (r-0.691, - 0.695, P<0.05). The level of miRNA-206 and miRNA-155 in patients with relapse was significantly lower than that in patients without relapse (P<0.05). ROC curve analysis showed that the area under the curve of miRNA-206 was 0.752 (95% CI=0.628-0.849), the sensitivity and specificity of diagnosing hyperthyroidism recurrence at the best critical value were 82.54% and 69.41%, AUC of miRNA-155 was 0.749 (95%CI=0.619-0.852), and the sensitivity and specificity of diagnosing hyperthyroidism recurrence at the best critical value were 84.26% and 65.28%, respectively. 

Conclusion: The levels of miRNA-206 and miRNA-155 in the serum of hyperthyroidism patients are related to the thyroid hormone and have a certain importance for predicting the recurrence of hyperthyroidism.