MICRORNA-638 INHIBITED CELL PROLIFERATION BY TARGETING CDK2

Suo-Ni Li¹, Jie-Qun Ma¹, Qi Zheng¹, Yang Zhai¹, Jing Zhou¹, Zi-Jun Liao¹, Yu Yao^{2, *}

¹Department of Oncology, Shaanxi Provincial Tumor Hospital, No.309, Yan-Ta West Road, Xi’an 710061, Shaanxi, P.R. China. - ²Department of Oncology, the first affiliated hospital of Xi’an Jiaotong University, No.227, Yan-Ta West Road, Xi’an 710061, Shaanxi, P.R. China

Gastric cancer ranks as the fourth most common cancer, but the treatment strategies have had no breakthroughs for a long time. Gastric cancer has a high mortality rate. Recently, studies found that miRNAs could regulate the expression of gastric cancer’s target genes, thus influencing the malignant biological behaviour of tumour cells. miR-638 is a member of miRNAs, which has low expression in gastric carcinoma tissue samples. Through TargetScan, we found that CDK2 was one target gene of miR-638. After transfection, we used the methods of qRT-PCR, CCK-8 assay, flow cytometry, colony formation, western-bolt and dual-luciferase reporter assay to analyse the relationships and proved that miR-638 could act as a tumour suppressor gene to inhibit the proliferation of gastric cancer cells by targeting CDK2. Upregulating the expression of miR-638 may be a new method for the clinical treatment of gastric cancer. 

Through this project, we hope that we could make clear the relationship between miR-638 and CDK2, and at the same time, provide new ideas for gastric cancer treatment.