MIR-134-5P REGULATES MIGRATION AND INVASION OF LIVER CANCER CELLS THROUGH ITGB8

Wang Peng^{1, *}, Zhao Peng², Yang Chunyan³

¹Department of Oncology, Binzhou people’s Hospital, China, 256610 - ²Department of thyroid surgery, Binzhou people’s Hospital, China, 256610 - ³Department of thyroid surgery, Binzhou people’s Hospital, China, 256610

Objective: To investigate the effect of miR-134-5p on the migration and invasion of liver cancer (LC) cells through regulating ITGB8. 

Methods: A total of 68 patients with LC admitted to our hospital from May 2016 to May 2018 were enrolled in the research group (RG), and another 65 healthy subjects with concurrent physical examination were selected as the control group (CG) for prospective analysis. The expression levels of miR-134-5p and ITGB8 in peripheral blood of the two groups were detected, and the clinical value of miR-134-5p in LC was analyzed. In addition, LC cells were purchased for biological function testing. 

Results: The expression levels of miR-134-5p and ITGB8 in the peripheral blood of the RG were higher than those of the CG (P<0.050). The ROC curve analysis showed that miR-134-5p had a good predictive value for the occurrence of LC. After the transfection of miR-134-5p-mimics (overexpression sequence), the proliferation, invasion and migration ability of LC cells were decreased, and the apoptosis and Caspase-3 and Caspase-9 proteins were increased (P<0.050). While conversely, the transfection of sh-ITGB8 (overexpression sequence) brought markedly elevated proliferation, apoptosis and invasion ability, as well as reduced apoptosis and Caspase-3 and Caspase-9 proteins of LC cells (P<0.050). The dual fluorescein reporter enzyme demonstrated that ITGB8 was the target gene of miR-134-5p. 

Conclusion: MiR-134-5p was lowly expressed in LC, and it could inhibit the proliferation, migration and invasion of LC cells through targeted regulation of ITGB8.