EXPLORATION ON THE MECHANISM OF HSA-MIR-877-3P IN THE REGULATION OF THE CELL BIOLOGICAL BEHAVIOR OF BREAST CANCER CELLS BY PARTICIPATING IN HER-2 SIGNALING PATHWAY

Guoxiang Zeng^{1, #}, Shenglong Guo^{2, #}, Mi Tian³, Lili He⁴, Xin Li^{4, *}

¹Departmen of General Surgery, The People’s Hospital of Hanchuan City, Hanchuan, PR China - ²Department of General Surgery, The Second People's Hospital of Hubei Province, Jingmen, PR China - ³Forensic Identification Center of Jingmen Public Security Bureau, Jingmen, PR China - ⁴Department of Thyroid and Breast Surgery, The Second People's Hospital of Hubei Province, Jingmen, PR China

Objective: To analyse the mechanism of hsa-miR-877-3p in the Regulation of the Cell Biological Behaviour of Breast Cancer Cells by Participating in HER-2 Signalling Pathway. 

Methods: The human breast cancer cell line MCF-7 with a low HER-2 expression was cultured in vitro, with the human breast cancer cell line HER-2/MCF-7 being constructed by overexpressing HER-2 transfected MCF-7 cells. Expression of hsa-miR-877-3p in MCF-7 cells and HER-2/MCF-7 cells was detected using real-time quantitative PCR; HER-2/MCF-7 cells transfected with an overexpression of hsa-miR-877-3p were used as the transfection group, and HER-2/MCF-7 cells transfected with empty plasmids were the control group. The cell biological behaviours of the two groups were detected through a scratch test, MTT, and Transwell experiments.

Results: The expression of hsa-miR-877-3p in HER-2/MCF-7 cells were significantly lower than that in MCF-7 cells, and the difference was statistically significant (P<0.05); the results of the scratch test showed that the scratch healing rate after the HER-2/MCF-7 cells was transfected with hsa-miR-877-3p was significantly slower than that of the control group, and the difference was statistically significant (P<0.05). The results of the MTT assay showed that the proliferation after HER-2/MCF-7 cells were transfected with hsa-miR-877-3p was significantly lower than that of the control group (P<0.05), and the difference was statistically significant (P<0.05); The results of the MTT assay showed that the proliferation of the HER-2/MCF-7 cells which were transfected with hsa-miR-877-3p was significantly lower than that of the control group (P<0.05), and the difference was statistically significant (P<0.05). 

Conclusion: The hsa-miR-877-3p expression is obviously down-regulated in human breast cancer HER-2/MCF-7 cells, and this can significantly inhibit the proliferation, invasion, and migration of HER-2 positive breast cancer cells. Consequently, this can be a new target for the treatment of breast cancer.