COMPREHENSIVE MULTIVARIATE ANALYSIS OF THE EFFECT OF DBL ON LENS EPITHELIAL CELLS

Sun Fenghua¹, Zhang Kun², Du Xiaoyan^{3, *}

¹Department of Psychology, People's Hospital of Gaomi in Shandong Province, Gaomi, 261500, China - ²Department of Geriatrics, People's Hospital of Gaomi in Shandong Province, Gaomi, 261500, China - ³Department of Ophthalmology, People's Hospital of Gaomi in Shandong Province, Gaomi, 261500, China

Background: The lens epithelial cells located in anterior subcapsular and lens equator subcapsular will cause cataracts, if it over proliferated. Therefore, understanding the factors affecting the proliferation of lens epithelial cells may have a positive effect on improving the diagnosis and treatment of cataract. However, we knew a little about the proliferation of lens epithelial cells.

Objective: Therefore, this study aims to investigate the effects of non-coding RNA and transcription factors in the proliferation of lens epithelial cells.

Methord: We synthesized differential analysis, co-expression analysis, enrichment analysis and hypergeometric prediction to identify the key factors affecting lens epithelial cells. In the end, we obtained three differentially expressed genes in the inflammatory response of lens epithelial cells in different periods. Three overlapping genes were observed. The multiple actions of these genes in different progressions were considered to be the key gene. Then, relevant modules for the disease were obtained based on co-expression and associated phenotypic analysis. Functional and pathway enrichment results underlined the effects of cell proliferation, growth, DNA damage, and the function of the dysregulation of PI3K, Akt, Notch pathways in the proliferation of lens epithelial cells. 

Result: We identified a series of ncRNAs and transcription factors that activate the activation or inhibition of the dysfunctional module by the pivot regulator. 

Conclusion: The analysis reveals that a potential pathogenic mechanism of lens epithelial cell proliferation and a dysfunction block analysis of lens epithelial cell proliferation. We further identified NCRNAs such as E530011L22Rik, Snora65 and microRNA-1192, and transcription factors such as Fos and Stat1, which are potential factors affecting the proliferation of lens epithelial cells. These results are helpful for the diagnosis and treatment of diseases related to lens epithelial cell proliferation.