THE ROLE OF LONG-CHAIN NON-CODING RNA EGFR-AS1 IN THE DEVELOPMENT AND METASTASIS OF RENAL CARCINOMA

Yang Wang, Junhong Li, Xilong Wang, Wandong Yu, Yangyun Wang1 Jun Zhang, Guowei Shi#

Department of Urologic, Shanghai NO.5 People’s Hospital, Fudan University, Shanghai, PR China

Objective: To investigate the effects of long-chain non-coding RNA EGFR-AS1 on the development and metastasis of renal cell carcinoma. 

Methods: EGFR-AS1 expression was detected in samples taken from 40 normal patients, comprising 40 renal parenchyma specimens and 40 renal carcinoma samples. The relationships between the expression of EGFR-AS1 in kidney cancer tissues and age, sex, tissue progression, TNM staging, and lymph node metastasis were analyzed. In vitro cell assay was conducted. EGFR-AS1 small interfering RNA was used to down-regulate the expression of EGFR-AS1 in renal cancer cells. Transwell assay was used to detect the invasive ability of interfering and undisturbed renal cancer cells. Cell scratch test was used to detect migration ability changes after EGFR-AS1 expression down-regulation in renal cancer cells. 

Results: In normal human kidney tissues, the expression of EGFR-AS1 was lower, while the expression level of EGFR-AS1 was higher in renal carcinoma. After analysis, it was found that the expression of EGFR-AS1 was significantly correlated with the TNM stage of renal carcinoma and whether lymph node metastasis had occurred (P<0.05). Transwell experiments showed that the transmembrane cell count of renal cancer cells after small interfering RNA interference was lower than that without interference (P<0.05); the cell scratch test showed the migration distance of small interference RNA in renal cancer cells after interference was shorter than that without interference.

Conclusion: EGFR-AS1 is highly expressed in human renal carcinoma tissues and promotes the proliferation and infiltration of tumour cells.