STUDY ON THE CHANGES OF TGA, TMA, TRAB, 25-HYDROXYVITAMIN-D AND INFLAMMATORY FACTORS IN PATIENTS WITH HYPERTHYROIDISM

Yinlu Tang, Siqi Zheng, Liping Fan, Yechen Chu, Yunya Yu, Xiaoyun Xie#

Jiaxing University, Jiaxing, PR China

Objective: To investigate the changes of thyroglobulin antibody (TGA), thyroid microsomal antibody (TMA), thyrotropin receptor antibody (TRAb), 25-hydroxyvitamin-D (25 (OH) D) and inflammatory factors in patients with hyperthyroidism.

Methods: Fifty-two hyperthyroidism patients who received treatment in the Department of Endocrinology at our hospital from July 2016 to January 2018 were randomly selected as the observation group, while 50 healthy subjects who came to our hospital for an examination at the same time were selected as the control group. To start, 4 ml of venous blood from the median cubital vein was extracted from all participants in the morning, while the levels of TGA, TMA, TRAb were detected by radioimmunity method. The 25-(OH) D level was determined by the chemiluminescence method and the level of GPT and GOT was measured by an automatic biochemical analyzer. The levels of TNF-α, IL-6 and Interleukin-2 (IL-2) were measured by enzyme-linked immunosorbent assay (ELISA), while the levels of free triiodothyronine (FT3), free thyroxine (FT4) and thyroid stimulating hormone (TSH) in the two groups were determined by the chemiluminescence method.

Results: The results showed that the levels of TGA, TMA and TRAb in the observation group were significantly higher than that in the control group (P<0.01). The level of GPT and GOT in the observation group was remarkably higher than that in the control group, while the level of 25-(OH) D in the observation group was significantly lower than that of the control group. Moreover, the levels of TNF-α, IL-6 and IL-2 in the observation group were higher than those in the control group (P<0.01). The level of FT3 and FT4 in the observation group was significantly higher than that in the control group, while the level of TSH in the observation group was markedly lower than that in the control group (P<0.01).

Conclusion: The level of 25-hydroxyvitamin D in patients with hyperthyroidism was significantly lower than that in healthy subjects, and TGA, TMA and TRAb levels were significantly higher than that in healthy subjects. This provided an important basis for the diagnosis and treatment of thyroid diseases. Furthermore, the immunosuppression of hyperthyroidism patients is significantly enhanced, which will damage the immune function of T cells, and then lead to inflammatory response.